Structure and distribution of a broadly expressed atypical sodium channel

Armen N Akopian, Veronika Souslova, Lucia Sivilotti, John N. Wood

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

A cDNA clone isolated from a rat dorsal root ganglion library encodes a 195 kDa voltage-gated sodium channel-like protein (SCL-11) with homology to the mouse (87%) and human (72%) atypical Na+ channels and rat partial clone NaG (98%). Two dominant mRNAs of 4.5 and 7 kb are expressed. The transcripts are present in lung, Schwann cells, pituitary and tissues containing smooth muscle cells. No functional channels could be detected on oocyte injection with cRNA, consistent with the absence of structural features necessary for voltage-gated sodium channel activity.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalFEBS Letters
Volume400
Issue number2
DOIs
StatePublished - Jan 3 1997
Externally publishedYes

Fingerprint

Voltage-Gated Sodium Channels
Sodium Channels
Rats
Clone Cells
Cells
Complementary RNA
Schwann Cells
Spinal Ganglia
Smooth Muscle Myocytes
Oocytes
Muscle
Complementary DNA
Tissue
Lung
Messenger RNA
Injections
Proteins

Keywords

  • cDNA cloning
  • Dorsal root ganglion
  • Voltage-gated sodium channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structure and distribution of a broadly expressed atypical sodium channel. / Akopian, Armen N; Souslova, Veronika; Sivilotti, Lucia; Wood, John N.

In: FEBS Letters, Vol. 400, No. 2, 03.01.1997, p. 183-187.

Research output: Contribution to journalArticle

Akopian, Armen N ; Souslova, Veronika ; Sivilotti, Lucia ; Wood, John N. / Structure and distribution of a broadly expressed atypical sodium channel. In: FEBS Letters. 1997 ; Vol. 400, No. 2. pp. 183-187.
@article{c34de9d509e8462b8e51ab9649d4188f,
title = "Structure and distribution of a broadly expressed atypical sodium channel",
abstract = "A cDNA clone isolated from a rat dorsal root ganglion library encodes a 195 kDa voltage-gated sodium channel-like protein (SCL-11) with homology to the mouse (87{\%}) and human (72{\%}) atypical Na+ channels and rat partial clone NaG (98{\%}). Two dominant mRNAs of 4.5 and 7 kb are expressed. The transcripts are present in lung, Schwann cells, pituitary and tissues containing smooth muscle cells. No functional channels could be detected on oocyte injection with cRNA, consistent with the absence of structural features necessary for voltage-gated sodium channel activity.",
keywords = "cDNA cloning, Dorsal root ganglion, Voltage-gated sodium channel",
author = "Akopian, {Armen N} and Veronika Souslova and Lucia Sivilotti and Wood, {John N.}",
year = "1997",
month = "1",
day = "3",
doi = "10.1016/S0014-5793(96)01389-0",
language = "English (US)",
volume = "400",
pages = "183--187",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Structure and distribution of a broadly expressed atypical sodium channel

AU - Akopian, Armen N

AU - Souslova, Veronika

AU - Sivilotti, Lucia

AU - Wood, John N.

PY - 1997/1/3

Y1 - 1997/1/3

N2 - A cDNA clone isolated from a rat dorsal root ganglion library encodes a 195 kDa voltage-gated sodium channel-like protein (SCL-11) with homology to the mouse (87%) and human (72%) atypical Na+ channels and rat partial clone NaG (98%). Two dominant mRNAs of 4.5 and 7 kb are expressed. The transcripts are present in lung, Schwann cells, pituitary and tissues containing smooth muscle cells. No functional channels could be detected on oocyte injection with cRNA, consistent with the absence of structural features necessary for voltage-gated sodium channel activity.

AB - A cDNA clone isolated from a rat dorsal root ganglion library encodes a 195 kDa voltage-gated sodium channel-like protein (SCL-11) with homology to the mouse (87%) and human (72%) atypical Na+ channels and rat partial clone NaG (98%). Two dominant mRNAs of 4.5 and 7 kb are expressed. The transcripts are present in lung, Schwann cells, pituitary and tissues containing smooth muscle cells. No functional channels could be detected on oocyte injection with cRNA, consistent with the absence of structural features necessary for voltage-gated sodium channel activity.

KW - cDNA cloning

KW - Dorsal root ganglion

KW - Voltage-gated sodium channel

UR - http://www.scopus.com/inward/record.url?scp=0031022041&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031022041&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(96)01389-0

DO - 10.1016/S0014-5793(96)01389-0

M3 - Article

VL - 400

SP - 183

EP - 187

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -