Structure and chromosomal mapping of the mouse P2X3 gene

Veronika Souslova, Samantha Ravenall, Margaret Fox, Dagan Wells, John N. Wood, Armen N. Akopian

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

P2X3 is one of seven cloned ATP-gated non-selective cation channels. We have isolated a full-length mouse P2X3 gene from a phage λ-129/Sv genomic library. The gene consists of 12 exons spanning a locus of approximately 40 kb. No significant similarities have been found between the genomic organisation of the mouse P2X3 gene and genes encoding other ion channels. The encoded mouse P2X3 protein consists of 397 amino acids and shows 99% identity with rat P2X3. Using RNase protection and primer extension assays, multiple transcription initiation sites have been mapped in the mouse P2X3 promoter to a region 162-168 bp upstream of the translation initiation codon. The P2X3 gene has been mapped to mouse chromosome 2p by fluorescence in situ hybridisation. The RAG locus-associated gene T160 is located 1.8 kb upstream of the transcription start site of mouse P2X3 gene. The promoter region of the mouse P2X3 gene lacks a conventional TATA and CCAAT consensus sites, and initiator elements. P2X3 is the first member of the P2X3 gene family to be completely characterised.

Original languageEnglish (US)
Pages (from-to)101-111
Number of pages11
JournalGene
Volume195
Issue number1
DOIs
StatePublished - Aug 11 1997
Externally publishedYes

Keywords

  • FISH
  • Genomic clone
  • Ion channel
  • Neuron

ASJC Scopus subject areas

  • Genetics

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