Structure and chromosomal localization of the human lymphotoxin gene

Glenn E. Nedwin, Julie Jarrett‐Nedwin, Douglas H. Smith, Susan L. Naylor, Alan Y. Sakaguchi, David V. Goeddel, Patrick W. Gray

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

We have isolated, sequenced, and determined the chromosomal localization of the gene encoding human lymphotoxin (LT). The single copy gene was isolated from a human genomic library using a 32P‐labeled 116 bp synthetic DNA fragment whose sequence was based on the NH2‐terminal amino acid sequence of LT. The gene spans 3 kb of DNA and is interrupted by three intervening sequences. The LT gene is located on human chromosome 6, as determined by Southern blot analysis of human‐murine hybrid DNA. Putative transcriptional control regions and areas of homology with the promoters of interferon and other genes are identified.

Original languageEnglish (US)
Pages (from-to)171-181
Number of pages11
JournalJournal of Cellular Biochemistry
Volume29
Issue number3
DOIs
StatePublished - 1985

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Keywords

  • chromosome 6
  • cytotoxic factor
  • gene structure
  • lymphotoxin

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nedwin, G. E., Jarrett‐Nedwin, J., Smith, D. H., Naylor, S. L., Sakaguchi, A. Y., Goeddel, D. V., & Gray, P. W. (1985). Structure and chromosomal localization of the human lymphotoxin gene. Journal of Cellular Biochemistry, 29(3), 171-181. https://doi.org/10.1002/jcb.240290302