Structure and Activities of the Eukaryotic RNA Exosome

Elizabeth V. Wasmuth, Christopher D. Lima

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

The composition of the multisubunit eukaryotic RNA exosome was described more than a decade ago, and structural studies conducted since that time have contributed to our mechanistic understanding of factors that are required for 3'-to-5' RNA processing and decay. This chapter describes the organization of the eukaryotic RNA exosome with a focus on presenting results related to the noncatalytic nine-subunit exosome core as well as the hydrolytic exo- and endoribonuclease Rrp44 (Dis3) and the exoribonuclease Rrp6. This is achieved in large part by describing crystal structures of Rrp44, Rrp6, and the nine-subunit exosome core with an emphasis on how these molecules interact to endow the RNA exosome with its catalytic activities.

Original languageEnglish (US)
Title of host publicationEnzymes
PublisherAcademic Press
Pages53-75
Number of pages23
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameEnzymes
Volume31
ISSN (Print)1874-6047

Keywords

  • 3'-to-5' RNA decay
  • Cold-shock domain
  • Endoribonuclease
  • Exoribonuclease
  • Exosome
  • Hydrolytic
  • KH domain
  • Multisubunit
  • PH domain
  • Phosphorolytic
  • PIN domain
  • RNA binding
  • RNA degradation
  • RNA processing
  • RNase
  • S1 domain
  • Structure

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biochemistry
  • Molecular Biology

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