Structure and Activities of the Eukaryotic RNA Exosome

Elizabeth V. Wasmuth, Christopher D. Lima

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations


The composition of the multisubunit eukaryotic RNA exosome was described more than a decade ago, and structural studies conducted since that time have contributed to our mechanistic understanding of factors that are required for 3'-to-5' RNA processing and decay. This chapter describes the organization of the eukaryotic RNA exosome with a focus on presenting results related to the noncatalytic nine-subunit exosome core as well as the hydrolytic exo- and endoribonuclease Rrp44 (Dis3) and the exoribonuclease Rrp6. This is achieved in large part by describing crystal structures of Rrp44, Rrp6, and the nine-subunit exosome core with an emphasis on how these molecules interact to endow the RNA exosome with its catalytic activities.

Original languageEnglish (US)
Title of host publicationEnzymes
PublisherAcademic Press
Number of pages23
StatePublished - 2012
Externally publishedYes

Publication series

ISSN (Print)1874-6047


  • 3'-to-5' RNA decay
  • Cold-shock domain
  • Endoribonuclease
  • Exoribonuclease
  • Exosome
  • Hydrolytic
  • KH domain
  • Multisubunit
  • PH domain
  • PIN domain
  • Phosphorolytic
  • RNA binding
  • RNA degradation
  • RNA processing
  • RNase
  • S1 domain
  • Structure

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biochemistry
  • Molecular Biology


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