Structural organization, mapping, characterization and evolutionary relationships of CDKN2 gene family members in Xiphophorus fishes

Steven Kazianis, Vandeeta A. Khanolkar, Rodney S. Nairn, J. Douglas Rains, David Trono, Rachel Garcia, Earlanda L. Williams, Ronald B. Walter

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Xiphophorus fishes and their hybrids are used as models for the study of melanoma and other diseases. The cyclin-dependent kinase inhibitor gene family in humans is comprised of four members, including CDKN2A (P16), and dysregulation of this gene is implicated in numerous neoplasms including melanomas. We have investigated the status of the gene family in the southern platyfish X. maculatus. Xiphophorus harbors at least two such loci, which we now term CDKN2A/B and CDKN2D. Both loci map to Xiphophorus linkage group 5, a genomic area that has long been known to harbor the DIFF tumor suppressor locus. Within this report, we report on the complete cloning, genomic exon/intron boundary delineation, linkage mapping and expressional characteristics of Xiphophorus CDKN2D. We also compare and contrast this expression to that of the previously isolated CDKN2AB locus in normal and neoplastic tissues derived from non-hybrid and hybrid fishes. The hypothetical evolutionary relationships of gene family members and their involvement in melanoma is evaluated. In comparison to CDKN2A/B, the RNA expression of Xiphophorus CDKN2D differs in normal tissues and is not associated with melanotic/pathologic tissues, confirming functional divergence between obvious homologues.

Original languageEnglish (US)
Pages (from-to)291-299
Number of pages9
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume138
Issue number3
DOIs
StatePublished - Jul 2004
Externally publishedYes

Fingerprint

Cyprinodontiformes
p16 Genes
Fish
Genes
Tissue
Ports and harbors
Melanoma
Cyclin-Dependent Kinases
Cloning
Fishes
Introns
Tumors
Exons
Chromosome Mapping
RNA
Organism Cloning
Neoplasms

Keywords

  • Cell cycle
  • INK4
  • Melanoma
  • Platyfish
  • Swordtail
  • Teleost

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Structural organization, mapping, characterization and evolutionary relationships of CDKN2 gene family members in Xiphophorus fishes. / Kazianis, Steven; Khanolkar, Vandeeta A.; Nairn, Rodney S.; Rains, J. Douglas; Trono, David; Garcia, Rachel; Williams, Earlanda L.; Walter, Ronald B.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 138, No. 3, 07.2004, p. 291-299.

Research output: Contribution to journalArticle

Kazianis, Steven ; Khanolkar, Vandeeta A. ; Nairn, Rodney S. ; Rains, J. Douglas ; Trono, David ; Garcia, Rachel ; Williams, Earlanda L. ; Walter, Ronald B. / Structural organization, mapping, characterization and evolutionary relationships of CDKN2 gene family members in Xiphophorus fishes. In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology. 2004 ; Vol. 138, No. 3. pp. 291-299.
@article{8563328bd63244d58bfc5d99b72d9549,
title = "Structural organization, mapping, characterization and evolutionary relationships of CDKN2 gene family members in Xiphophorus fishes",
abstract = "Xiphophorus fishes and their hybrids are used as models for the study of melanoma and other diseases. The cyclin-dependent kinase inhibitor gene family in humans is comprised of four members, including CDKN2A (P16), and dysregulation of this gene is implicated in numerous neoplasms including melanomas. We have investigated the status of the gene family in the southern platyfish X. maculatus. Xiphophorus harbors at least two such loci, which we now term CDKN2A/B and CDKN2D. Both loci map to Xiphophorus linkage group 5, a genomic area that has long been known to harbor the DIFF tumor suppressor locus. Within this report, we report on the complete cloning, genomic exon/intron boundary delineation, linkage mapping and expressional characteristics of Xiphophorus CDKN2D. We also compare and contrast this expression to that of the previously isolated CDKN2AB locus in normal and neoplastic tissues derived from non-hybrid and hybrid fishes. The hypothetical evolutionary relationships of gene family members and their involvement in melanoma is evaluated. In comparison to CDKN2A/B, the RNA expression of Xiphophorus CDKN2D differs in normal tissues and is not associated with melanotic/pathologic tissues, confirming functional divergence between obvious homologues.",
keywords = "Cell cycle, INK4, Melanoma, Platyfish, Swordtail, Teleost",
author = "Steven Kazianis and Khanolkar, {Vandeeta A.} and Nairn, {Rodney S.} and Rains, {J. Douglas} and David Trono and Rachel Garcia and Williams, {Earlanda L.} and Walter, {Ronald B.}",
year = "2004",
month = "7",
doi = "10.1016/j.cca.2004.08.003",
language = "English (US)",
volume = "138",
pages = "291--299",
journal = "Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology",
issn = "0742-8413",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Structural organization, mapping, characterization and evolutionary relationships of CDKN2 gene family members in Xiphophorus fishes

AU - Kazianis, Steven

AU - Khanolkar, Vandeeta A.

AU - Nairn, Rodney S.

AU - Rains, J. Douglas

AU - Trono, David

AU - Garcia, Rachel

AU - Williams, Earlanda L.

AU - Walter, Ronald B.

PY - 2004/7

Y1 - 2004/7

N2 - Xiphophorus fishes and their hybrids are used as models for the study of melanoma and other diseases. The cyclin-dependent kinase inhibitor gene family in humans is comprised of four members, including CDKN2A (P16), and dysregulation of this gene is implicated in numerous neoplasms including melanomas. We have investigated the status of the gene family in the southern platyfish X. maculatus. Xiphophorus harbors at least two such loci, which we now term CDKN2A/B and CDKN2D. Both loci map to Xiphophorus linkage group 5, a genomic area that has long been known to harbor the DIFF tumor suppressor locus. Within this report, we report on the complete cloning, genomic exon/intron boundary delineation, linkage mapping and expressional characteristics of Xiphophorus CDKN2D. We also compare and contrast this expression to that of the previously isolated CDKN2AB locus in normal and neoplastic tissues derived from non-hybrid and hybrid fishes. The hypothetical evolutionary relationships of gene family members and their involvement in melanoma is evaluated. In comparison to CDKN2A/B, the RNA expression of Xiphophorus CDKN2D differs in normal tissues and is not associated with melanotic/pathologic tissues, confirming functional divergence between obvious homologues.

AB - Xiphophorus fishes and their hybrids are used as models for the study of melanoma and other diseases. The cyclin-dependent kinase inhibitor gene family in humans is comprised of four members, including CDKN2A (P16), and dysregulation of this gene is implicated in numerous neoplasms including melanomas. We have investigated the status of the gene family in the southern platyfish X. maculatus. Xiphophorus harbors at least two such loci, which we now term CDKN2A/B and CDKN2D. Both loci map to Xiphophorus linkage group 5, a genomic area that has long been known to harbor the DIFF tumor suppressor locus. Within this report, we report on the complete cloning, genomic exon/intron boundary delineation, linkage mapping and expressional characteristics of Xiphophorus CDKN2D. We also compare and contrast this expression to that of the previously isolated CDKN2AB locus in normal and neoplastic tissues derived from non-hybrid and hybrid fishes. The hypothetical evolutionary relationships of gene family members and their involvement in melanoma is evaluated. In comparison to CDKN2A/B, the RNA expression of Xiphophorus CDKN2D differs in normal tissues and is not associated with melanotic/pathologic tissues, confirming functional divergence between obvious homologues.

KW - Cell cycle

KW - INK4

KW - Melanoma

KW - Platyfish

KW - Swordtail

KW - Teleost

UR - http://www.scopus.com/inward/record.url?scp=7944234073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7944234073&partnerID=8YFLogxK

U2 - 10.1016/j.cca.2004.08.003

DO - 10.1016/j.cca.2004.08.003

M3 - Article

C2 - 15533787

AN - SCOPUS:7944234073

VL - 138

SP - 291

EP - 299

JO - Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology

JF - Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology

SN - 0742-8413

IS - 3

ER -