Using P2Y1 specific oligonucleotide primers in a Polymerase Chain Reaction on human genomic DNA, we have amplified a region encoding the P2Y1 receptor. Restriction analysis and Southern hybridization of the PCR product revealed that the entire open reading frame of the human P2Y1 receptor is coded by an intronless gene. We have previously localized the P2Y1 receptor gene to human chromosome 3. The gene was further localized to a region of chromosome 3 using a subchromosomal hybrid panel containing different segments of chromosome 3. Based on the specific PCR product obtained and its Southern hybridization to the human P2Y1 receptor cRNA, the P2Y1 receptor gene was mapped to human chromosome 3q25.
ASJC Scopus subject areas
- Cell Biology