Structural characterization and fine chromosomal mapping of the human P2Y₁ purinergic receptor gene (P2RY₁)

Using P2Y₁ specific oligonucleotide primers in a Polymerase Chain Reaction on human genomic DNA, we have amplified a region encoding the P2Y₁ receptor. Restriction analysis and Southern hybridization of the PCR product revealed that the entire open reading frame of the human P2Y₁ receptor is coded by an intronless gene. We have previously localized the P2Y₁ receptor gene to human chromosome 3. The gene was further localized to a region of chromosome 3 using a subchromosomal hybrid panel containing different segments of chromosome 3. Based on the specific PCR product obtained and its Southern hybridization to the human P2Y₁ receptor cRNA, the P2Y₁ receptor gene was mapped to human chromosome 3q25.