Stressosomes formed in Bacillus subtilis from the RsbR protein of Listeria monocytogenes allow σB activation following exposure to either physical or nutritional stress

Luis Martinez, Adam Reeves, William Haldenwang

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The general stress regulon of Bacillus subtilis is controlled by σB, a transcription factor that is activated by physical or nutritional stress. In B. subtilis, each of these two stresses is communicated to the primary σB regulators by distinct pathways. Physical stress activation of σB involves a large-molecular-mass (>106-Da) structure (stressosome) formed by one or more homologous proteins (RsbRA, -B, -C, and -D) onto which the pathway's principal regulators are bound. The RsbR proteins are thought to be potential receptors for stress signaling. Listeria monocytogenes encodes orthologs of σB and its principal regulators; however, unlike B. subtilis, L. monocytogenes appears to use the stressosome pathway for both physical and nutritional stress activation of σB. In the current work, a B. subtilis strain that expressed L. monocytogenes rsbR (rsbRLm) in lieu of B. subtilis rsbR (rsbRBs) was created and was found to display the Listeria phenotype of σB activation following exposure to either physical or nutritional stress. B. subtilis expressing either the RsbR paralog rsbRC or rsbRD, but not rsbRA or rsbRB, as the sole source of RsbR also allowed σB induction following nutritional stress. It is unclear whether the nutritional stress induction seen in these strains is the result of a direct effect of nutritional stress on stressosome activity or a consequence of the background levels of σB activation in these strains and the effects of diminished ATP on the downstream phosphorylation reaction needed to reinactivate σB.

Original languageEnglish (US)
Pages (from-to)6279-6286
Number of pages8
JournalJournal of bacteriology
Volume192
Issue number23
DOIs
StatePublished - Dec 2010
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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