TY - JOUR
T1 - Stress resistance and aging
T2 - Influence of genes and nutrition
AU - Harper, James M.
AU - Salmon, Adam B.
AU - Chang, Yayi
AU - Bonkowski, Michael
AU - Bartke, Andrzej
AU - Miller, Richard A.
N1 - Funding Information:
We thank Gretchen Buehner, Maggie Vergara, Jessica Sewald and Alexis Xu for their technical assistance. This work was supported by NIH Grants AG16699, AG11687, and AG08808.
PY - 2006/8
Y1 - 2006/8
N2 - Previous studies have shown that dermal fibroblast cell lines derived from young adult mice of the long-lived Snell dwarf (d w / d w), Ames dwarf (df/df) and growth hormone receptor knockout (GHR-KO) mouse stocks are resistant, in vitro, to the cytotoxic effects of hydrogen peroxide, cadmium, ultraviolet light, paraquat, and heat. Here we show that, in contrast, fibroblasts from mice on low-calorie (CR) or low methionine (Meth-R) diets are not stress resistant in culture, despite the longevity induced by both dietary regimes. A second approach, involving induction of liver cell death in live animals using acetaminophen (APAP), documented hepatotoxin resistance in the CR and Meth-R mice, but d w / d w and GHR-KO mutant mice were not resistant to this agent, and were in fact more susceptible than littermate controls to the toxic effects of APAP. These data thus suggest that while resistance to stress is a common characteristic of experimental life span extension in mice, the cell types showing resistance may differ among the various models of delayed or decelerated aging.
AB - Previous studies have shown that dermal fibroblast cell lines derived from young adult mice of the long-lived Snell dwarf (d w / d w), Ames dwarf (df/df) and growth hormone receptor knockout (GHR-KO) mouse stocks are resistant, in vitro, to the cytotoxic effects of hydrogen peroxide, cadmium, ultraviolet light, paraquat, and heat. Here we show that, in contrast, fibroblasts from mice on low-calorie (CR) or low methionine (Meth-R) diets are not stress resistant in culture, despite the longevity induced by both dietary regimes. A second approach, involving induction of liver cell death in live animals using acetaminophen (APAP), documented hepatotoxin resistance in the CR and Meth-R mice, but d w / d w and GHR-KO mutant mice were not resistant to this agent, and were in fact more susceptible than littermate controls to the toxic effects of APAP. These data thus suggest that while resistance to stress is a common characteristic of experimental life span extension in mice, the cell types showing resistance may differ among the various models of delayed or decelerated aging.
KW - Caloric restriction
KW - Growth hormone receptor knockout
KW - Methionine restriction
KW - Snell dwarf
KW - Stress resistance
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U2 - 10.1016/j.mad.2006.04.002
DO - 10.1016/j.mad.2006.04.002
M3 - Article
C2 - 16713617
AN - SCOPUS:33745216272
SN - 0047-6374
VL - 127
SP - 687
EP - 694
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
IS - 8
ER -