Stress-induced changes in messenger RNA levels of N-methyl-d-aspartate and AMPA receptor subunits in selected regions of the rat hippocampus and hypothalamus

Viktor Bartanusz, J. M. Aubry, S. Pagliusi, D. Jezova, J. Baffi, J. Z. Kiss

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

The postsynaptic AMPA/kainate and N-methyl-d-aspartate-selective glutamate receptors are formed by zseveral different subunits and the overall subunit composition of the receptor appears to determine its physiological and pharmacological properties. Although glutamatergic mechanisms have been implicated in various forms of hippocampal stress responses, the impact of stress on glutamate receptor subunit composition has not yet been elucidated. We have used cell-by-cell quantitative in situ hybridization to assess stress-induced changes in transcript levels of N-methyl-d-aspartate and AMPA receptor subunit genes in subdivisions of the rat hippocampus and hypothalamus that are implicated in the stress response. We found that 24 h after a single immobilization stress there was a significant increase in the cellular level of NR1 subunit messenger RNA (about 35-45% above control values) in hippocampal CA3 and CA1 pyramidal cells as well as in neurons of the hypothalamic supraoptic and paraventricular nuclei. Moreover, in the CA3 area we have detected a concomitant increase (50% above controls) in the level of NR2B subunit messenger RNA, while the expression of NR2A subunit gene did not change after stress. Stress induced a selective decrease in the level of AMPA receptor subunit glutamate receptor A messenger RNA in neurons of both the CA3 and CA1 areas (18 and 24, respectively, below control values). These results suggest that the regulation of specific subunit messenger RNAs of the N-methyl-d-aspartate and AMPA receptors may be involved in altered hippocampal and hypothalamic responsiveness to glutamate and thus could play a critical role in stress-induced changes in their function.

Original languageEnglish (US)
Pages (from-to)247-252
Number of pages6
JournalNeuroscience
Volume66
Issue number2
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

AMPA Receptors
Hypothalamus
Hippocampus
Glutamate Receptors
Messenger RNA
Neurons
Supraoptic Nucleus
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Pyramidal Cells
Kainic Acid
Aspartic Acid
Immobilization
Genes
In Situ Hybridization
Glutamic Acid
Pharmacology
aspartic acid receptor

Keywords

  • alfa-amino-3-hydroxy-S-methyl-4-isoxazolepropionic acid
  • AMPA
  • N-methyl-d-aspartate
  • NMDA
  • paraventricular nucleus
  • PVN
  • SON
  • supraoptic nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Stress-induced changes in messenger RNA levels of N-methyl-d-aspartate and AMPA receptor subunits in selected regions of the rat hippocampus and hypothalamus. / Bartanusz, Viktor; Aubry, J. M.; Pagliusi, S.; Jezova, D.; Baffi, J.; Kiss, J. Z.

In: Neuroscience, Vol. 66, No. 2, 1995, p. 247-252.

Research output: Contribution to journalArticle

@article{5a17a10f5e7c4e01b53957f535123179,
title = "Stress-induced changes in messenger RNA levels of N-methyl-d-aspartate and AMPA receptor subunits in selected regions of the rat hippocampus and hypothalamus",
abstract = "The postsynaptic AMPA/kainate and N-methyl-d-aspartate-selective glutamate receptors are formed by zseveral different subunits and the overall subunit composition of the receptor appears to determine its physiological and pharmacological properties. Although glutamatergic mechanisms have been implicated in various forms of hippocampal stress responses, the impact of stress on glutamate receptor subunit composition has not yet been elucidated. We have used cell-by-cell quantitative in situ hybridization to assess stress-induced changes in transcript levels of N-methyl-d-aspartate and AMPA receptor subunit genes in subdivisions of the rat hippocampus and hypothalamus that are implicated in the stress response. We found that 24 h after a single immobilization stress there was a significant increase in the cellular level of NR1 subunit messenger RNA (about 35-45{\%} above control values) in hippocampal CA3 and CA1 pyramidal cells as well as in neurons of the hypothalamic supraoptic and paraventricular nuclei. Moreover, in the CA3 area we have detected a concomitant increase (50{\%} above controls) in the level of NR2B subunit messenger RNA, while the expression of NR2A subunit gene did not change after stress. Stress induced a selective decrease in the level of AMPA receptor subunit glutamate receptor A messenger RNA in neurons of both the CA3 and CA1 areas (18 and 24, respectively, below control values). These results suggest that the regulation of specific subunit messenger RNAs of the N-methyl-d-aspartate and AMPA receptors may be involved in altered hippocampal and hypothalamic responsiveness to glutamate and thus could play a critical role in stress-induced changes in their function.",
keywords = "alfa-amino-3-hydroxy-S-methyl-4-isoxazolepropionic acid, AMPA, N-methyl-d-aspartate, NMDA, paraventricular nucleus, PVN, SON, supraoptic nucleus",
author = "Viktor Bartanusz and Aubry, {J. M.} and S. Pagliusi and D. Jezova and J. Baffi and Kiss, {J. Z.}",
year = "1995",
doi = "10.1016/0306-4522(95)00084-V",
language = "English (US)",
volume = "66",
pages = "247--252",
journal = "Neuroscience",
issn = "0306-4522",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Stress-induced changes in messenger RNA levels of N-methyl-d-aspartate and AMPA receptor subunits in selected regions of the rat hippocampus and hypothalamus

AU - Bartanusz, Viktor

AU - Aubry, J. M.

AU - Pagliusi, S.

AU - Jezova, D.

AU - Baffi, J.

AU - Kiss, J. Z.

PY - 1995

Y1 - 1995

N2 - The postsynaptic AMPA/kainate and N-methyl-d-aspartate-selective glutamate receptors are formed by zseveral different subunits and the overall subunit composition of the receptor appears to determine its physiological and pharmacological properties. Although glutamatergic mechanisms have been implicated in various forms of hippocampal stress responses, the impact of stress on glutamate receptor subunit composition has not yet been elucidated. We have used cell-by-cell quantitative in situ hybridization to assess stress-induced changes in transcript levels of N-methyl-d-aspartate and AMPA receptor subunit genes in subdivisions of the rat hippocampus and hypothalamus that are implicated in the stress response. We found that 24 h after a single immobilization stress there was a significant increase in the cellular level of NR1 subunit messenger RNA (about 35-45% above control values) in hippocampal CA3 and CA1 pyramidal cells as well as in neurons of the hypothalamic supraoptic and paraventricular nuclei. Moreover, in the CA3 area we have detected a concomitant increase (50% above controls) in the level of NR2B subunit messenger RNA, while the expression of NR2A subunit gene did not change after stress. Stress induced a selective decrease in the level of AMPA receptor subunit glutamate receptor A messenger RNA in neurons of both the CA3 and CA1 areas (18 and 24, respectively, below control values). These results suggest that the regulation of specific subunit messenger RNAs of the N-methyl-d-aspartate and AMPA receptors may be involved in altered hippocampal and hypothalamic responsiveness to glutamate and thus could play a critical role in stress-induced changes in their function.

AB - The postsynaptic AMPA/kainate and N-methyl-d-aspartate-selective glutamate receptors are formed by zseveral different subunits and the overall subunit composition of the receptor appears to determine its physiological and pharmacological properties. Although glutamatergic mechanisms have been implicated in various forms of hippocampal stress responses, the impact of stress on glutamate receptor subunit composition has not yet been elucidated. We have used cell-by-cell quantitative in situ hybridization to assess stress-induced changes in transcript levels of N-methyl-d-aspartate and AMPA receptor subunit genes in subdivisions of the rat hippocampus and hypothalamus that are implicated in the stress response. We found that 24 h after a single immobilization stress there was a significant increase in the cellular level of NR1 subunit messenger RNA (about 35-45% above control values) in hippocampal CA3 and CA1 pyramidal cells as well as in neurons of the hypothalamic supraoptic and paraventricular nuclei. Moreover, in the CA3 area we have detected a concomitant increase (50% above controls) in the level of NR2B subunit messenger RNA, while the expression of NR2A subunit gene did not change after stress. Stress induced a selective decrease in the level of AMPA receptor subunit glutamate receptor A messenger RNA in neurons of both the CA3 and CA1 areas (18 and 24, respectively, below control values). These results suggest that the regulation of specific subunit messenger RNAs of the N-methyl-d-aspartate and AMPA receptors may be involved in altered hippocampal and hypothalamic responsiveness to glutamate and thus could play a critical role in stress-induced changes in their function.

KW - alfa-amino-3-hydroxy-S-methyl-4-isoxazolepropionic acid

KW - AMPA

KW - N-methyl-d-aspartate

KW - NMDA

KW - paraventricular nucleus

KW - PVN

KW - SON

KW - supraoptic nucleus

UR - http://www.scopus.com/inward/record.url?scp=0028947331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028947331&partnerID=8YFLogxK

U2 - 10.1016/0306-4522(95)00084-V

DO - 10.1016/0306-4522(95)00084-V

M3 - Article

C2 - 7477869

AN - SCOPUS:0028947331

VL - 66

SP - 247

EP - 252

JO - Neuroscience

JF - Neuroscience

SN - 0306-4522

IS - 2

ER -