Stranded whole transcriptome RNA-Seq for all RNA types

David F.B. Miller; Pearlly X. Yan; Fang Fang; Aaron Buechlein; James B. Ford; Haixu Tang; Tim H. Huang; Matthew E. Burow; Yunlong Liu; Douglas B. Rusch; Kenneth P. Nephew

doi:10.1002/0471142905.hg1114s84

Stranded whole transcriptome RNA-Seq for all RNA types

David F.B. Miller, Pearlly X. Yan, Fang Fang, Aaron Buechlein, James B. Ford, Haixu Tang, Tim H. Huang, Matthew E. Burow, Yunlong Liu, Douglas B. Rusch, Kenneth P. Nephew

Department of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA(Piwi-interacting RNA), snoRNA(small nucleolar RNA), lincRNA(large non-coding intergenicRNA), SRPRNA(signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.

Original language	English (US)
Pages (from-to)	11.14.1-11.14.23
Journal	Current Protocols in Human Genetics
Volume	2015
DOIs	https://doi.org/10.1002/0471142905.hg1114s84
State	Published - 2015

Keywords

Duplex-specific nuclease
Gene expression
RNA-Seq
Transcriptome

ASJC Scopus subject areas

Genetics(clinical)
Genetics

Access to Document

10.1002/0471142905.hg1114s84

Cite this

@article{7e3b4307a89f4f4c8028b2df24260095,

title = "Stranded whole transcriptome RNA-Seq for all RNA types",

abstract = "Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA(Piwi-interacting RNA), snoRNA(small nucleolar RNA), lincRNA(large non-coding intergenicRNA), SRPRNA(signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.",

keywords = "Duplex-specific nuclease, Gene expression, RNA-Seq, Transcriptome",

author = "Miller, {David F.B.} and Yan, {Pearlly X.} and Fang Fang and Aaron Buechlein and Ford, {James B.} and Haixu Tang and Huang, {Tim H.} and Burow, {Matthew E.} and Yunlong Liu and Rusch, {Douglas B.} and Nephew, {Kenneth P.}",

note = "Publisher Copyright: {\textcopyright} 2015 by John Wiley & Sons, Inc.",

year = "2015",

doi = "10.1002/0471142905.hg1114s84",

language = "English (US)",

volume = "2015",

pages = "11.14.1--11.14.23",

journal = "Current Protocols in Human Genetics",

issn = "1934-8266",

publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Stranded whole transcriptome RNA-Seq for all RNA types

AU - Miller, David F.B.

AU - Yan, Pearlly X.

AU - Fang, Fang

AU - Buechlein, Aaron

AU - Ford, James B.

AU - Tang, Haixu

AU - Huang, Tim H.

AU - Burow, Matthew E.

AU - Liu, Yunlong

AU - Rusch, Douglas B.

AU - Nephew, Kenneth P.

PY - 2015

Y1 - 2015

N2 - Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA(Piwi-interacting RNA), snoRNA(small nucleolar RNA), lincRNA(large non-coding intergenicRNA), SRPRNA(signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.

AB - Stranded whole transcriptome RNA-Seq described in this unit captures quantitative expression data for all types of RNA including, but not limited to, miRNA (microRNA), piRNA(Piwi-interacting RNA), snoRNA(small nucleolar RNA), lincRNA(large non-coding intergenicRNA), SRPRNA(signal recognition particle RNA), tRNA (transfer RNA), mtRNA (mitochondrial RNA), and mRNA (messenger RNA). The size and nature of these types of RNA are irrelevant to the approach described here. Barcoded libraries for multiplexing on the Illumina platform are generated with this approach but it can be applied to other platforms with a few modifications.

KW - Duplex-specific nuclease

KW - Gene expression

KW - RNA-Seq

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=84949294348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949294348&partnerID=8YFLogxK

U2 - 10.1002/0471142905.hg1114s84

DO - 10.1002/0471142905.hg1114s84

M3 - Article

C2 - 25599667

AN - SCOPUS:84949294348

SN - 1934-8266

VL - 2015

SP - 11.14.1-11.14.23

JO - Current Protocols in Human Genetics

JF - Current Protocols in Human Genetics

ER -

Stranded whole transcriptome RNA-Seq for all RNA types

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this