Storage of spheroplasts at -70°C for transfection with φX174 RFDNA

H. V. Malling; M. C. Fater; J. G. Burkhart; S. C. Hardies; C. A. Hutchison; M. H. Edgell

doi:10.1016/0735-0651(87)90014-8

Storage of spheroplasts at -70°C for transfection with φX174 RFDNA

H. V. Malling, M. C. Fater, J. G. Burkhart, S. C. Hardies, C. A. Hutchison, M. H. Edgell

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70°C.

Original language	English (US)
Pages (from-to)	23-26
Number of pages	4
Journal	Gene Analysis Techniques
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/0735-0651(87)90014-8
State	Published - Jan 1 1987

ASJC Scopus subject areas

Genetics

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title = "Storage of spheroplasts at -70°C for transfection with φX174 RFDNA",

abstract = "A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70°C.",

author = "Malling, {H. V.} and Fater, {M. C.} and Burkhart, {J. G.} and Hardies, {S. C.} and Hutchison, {C. A.} and Edgell, {M. H.}",

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AU - Fater, M. C.

AU - Burkhart, J. G.

AU - Hardies, S. C.

AU - Hutchison, C. A.

AU - Edgell, M. H.

PY - 1987/1/1

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