Storage of spheroplasts at -70°C for transfection with φX174 RFDNA

H. V. Malling, M. C. Fater, J. G. Burkhart, S. C. Hardies, C. A. Hutchison, M. H. Edgell

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A method was developed for preparation of spheroplasts used for transfection with φX174 RF DNA. This method had a high-level competence and retained the competence for up to one year of storage in 7% DMSO at -70°C.

Original languageEnglish (US)
Pages (from-to)23-26
Number of pages4
JournalGene Analysis Techniques
Volume4
Issue number2
DOIs
StatePublished - Jan 1 1987

ASJC Scopus subject areas

  • Genetics

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