Abstract
The physiological stimulation and inhibition of release of several peptide and protein hormones appears to be associated with transcription of the respective peptide- and protein hormone-encoding genes. In the current studies we investigated whether this was also true for the rat pituitary LH system. Using an anterior pituitary primary tissue culture system, we have analyzed the effects of 10-9 M GnRH on βLH gene transcription using a transcription run-on assay, and on nuclear βLH RNA levels using a highly sensitive solution hybridization-S1-nuclease protection assay. GnRH-stimulated release of LH does not appear to be coupled to a significant change in the rate of βLH gene transcription, but is associated with both a 2- to 3-fold increase in levels of the βLH primary transcript and processing intermediates and a rapid decrease in the levels of fully processed βLH mRNA in the nucleus. No significant change in βLH cytoplasmic mRNA levels, however, was associated with GnRH-stimulated release of LH, in vitro. Our findings suggest that unlike several other peptide and protein hormone systems, stimulated release of βLH by GnRH in vitro is not associated with an increase in βLH gene transcription or cytoplasmic mRNA levels.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1033-1042 |
| Number of pages | 10 |
| Journal | Molecular Endocrinology |
| Volume | 2 |
| Issue number | 11 |
| State | Published - 1988 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Molecular Biology
- Endocrinology, Diabetes and Metabolism
Cite this
Stimulation of pituitary luteinizing hormone secretion by gonadotropin-releasing hormone is not coupled to β-luteinizing hormone gene transcription. / Salton, S. R J; Blum, M.; Jonassen, J. A.; Clayton, R. N.; Roberts, James.
In: Molecular Endocrinology, Vol. 2, No. 11, 1988, p. 1033-1042.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stimulation of pituitary luteinizing hormone secretion by gonadotropin-releasing hormone is not coupled to β-luteinizing hormone gene transcription
AU - Salton, S. R J
AU - Blum, M.
AU - Jonassen, J. A.
AU - Clayton, R. N.
AU - Roberts, James
PY - 1988
Y1 - 1988
N2 - The physiological stimulation and inhibition of release of several peptide and protein hormones appears to be associated with transcription of the respective peptide- and protein hormone-encoding genes. In the current studies we investigated whether this was also true for the rat pituitary LH system. Using an anterior pituitary primary tissue culture system, we have analyzed the effects of 10-9 M GnRH on βLH gene transcription using a transcription run-on assay, and on nuclear βLH RNA levels using a highly sensitive solution hybridization-S1-nuclease protection assay. GnRH-stimulated release of LH does not appear to be coupled to a significant change in the rate of βLH gene transcription, but is associated with both a 2- to 3-fold increase in levels of the βLH primary transcript and processing intermediates and a rapid decrease in the levels of fully processed βLH mRNA in the nucleus. No significant change in βLH cytoplasmic mRNA levels, however, was associated with GnRH-stimulated release of LH, in vitro. Our findings suggest that unlike several other peptide and protein hormone systems, stimulated release of βLH by GnRH in vitro is not associated with an increase in βLH gene transcription or cytoplasmic mRNA levels.
AB - The physiological stimulation and inhibition of release of several peptide and protein hormones appears to be associated with transcription of the respective peptide- and protein hormone-encoding genes. In the current studies we investigated whether this was also true for the rat pituitary LH system. Using an anterior pituitary primary tissue culture system, we have analyzed the effects of 10-9 M GnRH on βLH gene transcription using a transcription run-on assay, and on nuclear βLH RNA levels using a highly sensitive solution hybridization-S1-nuclease protection assay. GnRH-stimulated release of LH does not appear to be coupled to a significant change in the rate of βLH gene transcription, but is associated with both a 2- to 3-fold increase in levels of the βLH primary transcript and processing intermediates and a rapid decrease in the levels of fully processed βLH mRNA in the nucleus. No significant change in βLH cytoplasmic mRNA levels, however, was associated with GnRH-stimulated release of LH, in vitro. Our findings suggest that unlike several other peptide and protein hormone systems, stimulated release of βLH by GnRH in vitro is not associated with an increase in βLH gene transcription or cytoplasmic mRNA levels.
UR - http://www.scopus.com/inward/record.url?scp=0023691551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023691551&partnerID=8YFLogxK
M3 - Article
C2 - 2464746
AN - SCOPUS:0023691551
VL - 2
SP - 1033
EP - 1042
JO - Molecular Endocrinology
JF - Molecular Endocrinology
SN - 0888-8809
IS - 11
ER -