Stimulation by insulin of RNA synthesis in chick fibroblasts

Joel B. Baseman, Domenic Paolini, Harold Amos

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

After the addition of insulin to monolayers of chick fibroblasts previously incubated in serum-free medium, the rates of protein and RNA synthesis increase continuously during the first 8-10 h. Little stimulation of DNA synthesis or mitosis results with the addition of insulin alone in contrast to the addition offresh serum which stimulates both markedly. The stimulation in RNA synthesis does not result from expansion of the nucleotide pool but is correlated with increases in RNA polymerase activity. All major classes of RNA are stimulated ; processing of preribosomal RNA to 28S and 18S and the association of this mature RNA with ribosomes appear to occur normally. The kinetics of stimulation of 5S RNA differ from those of the synthesis of 4S and of ribosomal RNA. Insulin and serum appear to affect the synthesis or stability of certain transcripts differentially.

Original languageEnglish (US)
Pages (from-to)54-64
Number of pages11
JournalJournal of Cell Biology
Volume60
Issue number1
DOIs
StatePublished - Jan 1 1974

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Stimulation by insulin of RNA synthesis in chick fibroblasts'. Together they form a unique fingerprint.

Cite this