@article{d3873b2c36c940a9959c9d8fde59a364,
title = "Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion",
abstract = "Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.",
keywords = "Dental stem cells, Differentiation, Inflammation, Oligodendrocyte progenitor cells, Spinal cord",
author = "{De Berdt}, Pauline and Pauline Bottemanne and John Bianco and Mireille Alhouayek and Anibal Diogenes and Amy Llyod and Jose Gerardo-Nava and Brook, {Gary A.} and V{\'e}ronique Miron and Muccioli, {Giulio G.} and Rieux, {Anne des}",
note = "Funding Information: Anne des Rieux is a Research Associate, Mireille Alhouayek is a Post-doctoral Researcher and Pauline Bottemanne is a FRIA Doctoral Researcher at the FRS-FNRS (Fonds de la Recherche Scientifique). The authors acknowledge Prof. O. Feron (UCL) for the access to hypoxia incubator and Daniel Soong (EdU) for his help with MBP quantification as well as Lo?c Germain (UCL) for his support in the development of the tri-cultures. We are also grateful to Universit? Catholique de Louvain (FSR) and?International Foundation for Research in Paraplegia (IRP) for the financial support. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. Funding Information: Acknowledgements Anne des Rieux is a Research Associate, Mireille Alhouayek is a Post-doctoral Researcher and Pauline Bottemanne is a FRIA Doctoral Researcher at the FRS-FNRS (Fonds de la Recherche Scientifique). The authors acknowledge Prof. O. Feron (UCL) for the access to hypoxia incubator and Daniel Soong (EdU) for his help with MBP quantification as well as Lo{\"i}c Germain (UCL) for his support in the development of the tri-cultures. We are also grateful to Univer-sit{\'e} Catholique de Louvain (FSR) and International Foundation for Research in Paraplegia (IRP) for the financial support. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article. Publisher Copyright: {\textcopyright} 2018, Springer International Publishing AG, part of Springer Nature.",
year = "2018",
month = aug,
day = "1",
doi = "10.1007/s00018-018-2764-5",
language = "English (US)",
volume = "75",
pages = "2843--2856",
journal = "Cellular and Molecular Life Sciences",
issn = "1420-682X",
publisher = "Birkhauser Verlag Basel",
number = "15",
}