Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

Pauline De Berdt, Pauline Bottemanne, John Bianco, Mireille Alhouayek, Anibal Diogenes, Amy Llyod, Jose Gerardo-Nava, Gary A. Brook, Véronique Miron, Giulio G. Muccioli, Anne des Rieux

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.

Original languageEnglish (US)
Pages (from-to)2843-2856
Number of pages14
JournalCellular and Molecular Life Sciences
Volume75
Issue number15
DOIs
StatePublished - Aug 1 2018

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Keywords

  • Dental stem cells
  • Differentiation
  • Inflammation
  • Oligodendrocyte progenitor cells
  • Spinal cord

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

De Berdt, P., Bottemanne, P., Bianco, J., Alhouayek, M., Diogenes, A., Llyod, A., Gerardo-Nava, J., Brook, G. A., Miron, V., Muccioli, G. G., & Rieux, A. D. (2018). Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion. Cellular and Molecular Life Sciences, 75(15), 2843-2856. https://doi.org/10.1007/s00018-018-2764-5