Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

Pauline De Berdt; Pauline Bottemanne; John Bianco; Mireille Alhouayek; Anibal Diogenes; Amy Llyod; Jose Gerardo-Nava; Gary A. Brook; Véronique Miron; Giulio G. Muccioli; Anne des Rieux

doi:10.1007/s00018-018-2764-5

Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

Pauline De Berdt, Pauline Bottemanne, John Bianco, Mireille Alhouayek, Anibal Diogenes, Amy Llyod, Jose Gerardo-Nava, Gary A. Brook, Véronique Miron, Giulio G. Muccioli, Anne des Rieux

Endodontics

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.

Original language	English (US)
Pages (from-to)	2843-2856
Number of pages	14
Journal	Cellular and Molecular Life Sciences
Volume	75
Issue number	15
DOIs	https://doi.org/10.1007/s00018-018-2764-5
State	Published - Aug 1 2018

Keywords

Dental stem cells
Differentiation
Inflammation
Oligodendrocyte progenitor cells
Spinal cord

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Pharmacology
Cellular and Molecular Neuroscience
Cell Biology

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De Berdt, P., Bottemanne, P., Bianco, J., Alhouayek, M., Diogenes, A., Llyod, A., Gerardo-Nava, J., Brook, G. A., Miron, V., Muccioli, G. G., & Rieux, A. D. (2018). Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion. Cellular and Molecular Life Sciences, 75(15), 2843-2856. https://doi.org/10.1007/s00018-018-2764-5

Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion. / De Berdt, Pauline; Bottemanne, Pauline; Bianco, John; Alhouayek, Mireille; Diogenes, Anibal; Llyod, Amy; Gerardo-Nava, Jose; Brook, Gary A.; Miron, Véronique; Muccioli, Giulio G.; Rieux, Anne des.

In: Cellular and Molecular Life Sciences, Vol. 75, No. 15, 01.08.2018, p. 2843-2856.

Research output: Contribution to journal › Article › peer-review

De Berdt, P, Bottemanne, P, Bianco, J, Alhouayek, M, Diogenes, A, Llyod, A, Gerardo-Nava, J, Brook, GA, Miron, V, Muccioli, GG & Rieux, AD 2018, 'Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion', Cellular and Molecular Life Sciences, vol. 75, no. 15, pp. 2843-2856. https://doi.org/10.1007/s00018-018-2764-5

De Berdt, Pauline ; Bottemanne, Pauline ; Bianco, John ; Alhouayek, Mireille ; Diogenes, Anibal ; Llyod, Amy ; Gerardo-Nava, Jose ; Brook, Gary A. ; Miron, Véronique ; Muccioli, Giulio G. ; Rieux, Anne des. / Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion. In: Cellular and Molecular Life Sciences. 2018 ; Vol. 75, No. 15. pp. 2843-2856.

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abstract = "Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.",

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note = "Funding Information: Acknowledgements Anne des Rieux is a Research Associate, Mireille Alhouayek is a Post-doctoral Researcher and Pauline Bottemanne is a FRIA Doctoral Researcher at the FRS-FNRS (Fonds de la Recherche Scientifique). The authors acknowledge Prof. O. Feron (UCL) for the access to hypoxia incubator and Daniel Soong (EdU) for his help with MBP quantification as well as Lo{\"i}c Germain (UCL) for his support in the development of the tri-cultures. We are also grateful to Univer-sit{\'e} Catholique de Louvain (FSR) and International Foundation for Research in Paraplegia (IRP) for the financial support. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.",

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AU - De Berdt, Pauline

AU - Bottemanne, Pauline

AU - Bianco, John

AU - Alhouayek, Mireille

AU - Diogenes, Anibal

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AU - Gerardo-Nava, Jose

AU - Brook, Gary A.

AU - Miron, Véronique

AU - Muccioli, Giulio G.

AU - Rieux, Anne des

N1 - Funding Information: Acknowledgements Anne des Rieux is a Research Associate, Mireille Alhouayek is a Post-doctoral Researcher and Pauline Bottemanne is a FRIA Doctoral Researcher at the FRS-FNRS (Fonds de la Recherche Scientifique). The authors acknowledge Prof. O. Feron (UCL) for the access to hypoxia incubator and Daniel Soong (EdU) for his help with MBP quantification as well as Loïc Germain (UCL) for his support in the development of the tri-cultures. We are also grateful to Univer-sité Catholique de Louvain (FSR) and International Foundation for Research in Paraplegia (IRP) for the financial support. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

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