A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.
|Original language||English (US)|
|Number of pages||10|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Sep 30 1987|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology