TY - JOUR
T1 - Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector
AU - Ghosh-Choudhury, Goutam
AU - Graham, Frank L.
PY - 1987/9/30
Y1 - 1987/9/30
N2 - A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.
AB - A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.
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U2 - 10.1016/S0006-291X(87)80164-X
DO - 10.1016/S0006-291X(87)80164-X
M3 - Article
C2 - 3663223
AN - SCOPUS:0023230477
VL - 147
SP - 964
EP - 973
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -