Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

Goutam Ghosh-Choudhury, Frank L. Graham

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.

Original languageEnglish (US)
Pages (from-to)964-973
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume147
Issue number3
DOIs
StatePublished - Sep 30 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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