Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

Goutam Ghosh-Choudhury; Frank L. Graham

doi:10.1016/S0006-291X(87)80164-X

Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

Goutam Ghosh-Choudhury, Frank L. Graham

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr⁺ cell line was established by stable transfer of the gene using the recombinant virus.

Original language	English (US)
Pages (from-to)	964-973
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	147
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(87)80164-X
State	Published - Sep 30 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector",

abstract = "A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.",

author = "Goutam Ghosh-Choudhury and Graham, {Frank L.}",

note = "Funding Information: Xhis work was supported by grants from the National Cancer Institute of Canada a~ Medical Research Council of Canada. F.L.G. is a Terry Fox Cancer Research Scientist of National Cancer Institute of Canada.",

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AU - Graham, Frank L.

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Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

Abstract

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