Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

Goutam Ghosh-Choudhury; Frank L. Graham

doi:10.1016/S0006-291X(87)80164-X

Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

Goutam Ghosh-Choudhury, Frank L. Graham

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr⁺ cell line was established by stable transfer of the gene using the recombinant virus.

Original language	English (US)
Pages (from-to)	964-973
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	147
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(87)80164-X
State	Published - Sep 30 1987
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/S0006-291X(87)80164-X

Cite this

@article{9273bde542b04ca8bc108b1f7b1e50f9,

title = "Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector",

abstract = "A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.",

author = "Goutam Ghosh-Choudhury and Graham, {Frank L.}",

note = "Funding Information: Xhis work was supported by grants from the National Cancer Institute of Canada a~ Medical Research Council of Canada. F.L.G. is a Terry Fox Cancer Research Scientist of National Cancer Institute of Canada.",

year = "1987",

month = sep,

day = "30",

doi = "10.1016/S0006-291X(87)80164-X",

language = "English (US)",

volume = "147",

pages = "964--973",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "3",

}

TY - JOUR

T1 - Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

AU - Ghosh-Choudhury, Goutam

AU - Graham, Frank L.

N1 - Funding Information: Xhis work was supported by grants from the National Cancer Institute of Canada a~ Medical Research Council of Canada. F.L.G. is a Terry Fox Cancer Research Scientist of National Cancer Institute of Canada.

PY - 1987/9/30

Y1 - 1987/9/30

N2 - A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.

AB - A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.

UR - http://www.scopus.com/inward/record.url?scp=0023230477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023230477&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(87)80164-X

DO - 10.1016/S0006-291X(87)80164-X

M3 - Article

C2 - 3663223

AN - SCOPUS:0023230477

VL - 147

SP - 964

EP - 973

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -

Stable transfer of a mouse dihydrofolate reductase gene into a deficient cell line using human adenovirus vector

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this