Abstract
A plasmid containing the mouse dihydrofolate reductase (dhfr) gene was rescued in a human adenovirus in early region 3. Analysis of the insert in the recombinant virus revealed that the dhfr sequences were intact in the viral genome, whereas a part of the ampicillin gene in the plasmid sequences was deleted. The recombinant virus could successfully express this gene in a deficient cell line. A permanent dhfr+ cell line was established by stable transfer of the gene using the recombinant virus.
Original language | English (US) |
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Pages (from-to) | 964-973 |
Number of pages | 10 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 147 |
Issue number | 3 |
DOIs | |
State | Published - Sep 30 1987 |
Externally published | Yes |
ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
- Cell Biology