TY - JOUR
T1 - Stability and in vitro DNA packaging of bacteriophages
T2 - Effects of dextrans, sugars, and polyols
AU - Serwer, P.
AU - Masker, W. E.
AU - Allen, J. L.
PY - 1983
Y1 - 1983
N2 - Attempts were made to increase the efficiency of infectious particle formation during the in vitro assembly of bacteriophage T7 from procapsids and DNA. It was found that dextrans and some smaller, related compounds (sucrose and sorbitol) increase this efficiency by a factor of 8 to 50. Dextrans also inhibited elevated temperature-induced emptying of DNA from bacteriophages T7, P22, and T4, suggesting that the stimulation of assembly is caused, at least in part, by the stabilization of packaged DNA in capsids. The data indicated that the sugars and polyols can slow DNA emptying from bacteriophages at elevated temperature whether they permeate the bacteriophage capsid or not. In contrast, the data suggested that permeation of some particle, probably a capsid, results in inhibition of in vitro T7 assembly.
AB - Attempts were made to increase the efficiency of infectious particle formation during the in vitro assembly of bacteriophage T7 from procapsids and DNA. It was found that dextrans and some smaller, related compounds (sucrose and sorbitol) increase this efficiency by a factor of 8 to 50. Dextrans also inhibited elevated temperature-induced emptying of DNA from bacteriophages T7, P22, and T4, suggesting that the stimulation of assembly is caused, at least in part, by the stabilization of packaged DNA in capsids. The data indicated that the sugars and polyols can slow DNA emptying from bacteriophages at elevated temperature whether they permeate the bacteriophage capsid or not. In contrast, the data suggested that permeation of some particle, probably a capsid, results in inhibition of in vitro T7 assembly.
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U2 - 10.1128/jvi.45.2.665-671.1983
DO - 10.1128/jvi.45.2.665-671.1983
M3 - Article
C2 - 6187934
AN - SCOPUS:0020666329
SN - 0022-538X
VL - 45
SP - 665
EP - 671
JO - Journal of virology
JF - Journal of virology
IS - 2
ER -