Stability and in vitro DNA packaging of bacteriophages: Effects of dextrans, sugars, and polyols

Philip Serwer, W. E. Masker, J. L. Allen

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Attempts were made to increase the efficiency of infectious particle formation during the in vitro assembly of bacteriophage T7 from procapsids and DNA. It was found that dextrans and some smaller, related compounds (sucrose and sorbitol) increase this efficiency by a factor of 8 to 50. Dextrans also inhibited elevated temperature-induced emptying of DNA from bacteriophages T7, P22, and T4, suggesting that the stimulation of assembly is caused, at least in part, by the stabilization of packaged DNA in capsids. The data indicated that the sugars and polyols can slow DNA emptying from bacteriophages at elevated temperature whether they permeate the bacteriophage capsid or not. In contrast, the data suggested that permeation of some particle, probably a capsid, results in inhibition of in vitro T7 assembly.

Original languageEnglish (US)
Pages (from-to)665-671
Number of pages7
JournalJournal of Virology
Volume45
Issue number2
StatePublished - 1983

Fingerprint

DNA Packaging
polyols
dextran
Dextrans
bacteriophages
Bacteriophages
capsid
Capsid
Bacteriophage T7
sugars
DNA
Bacteriophage P22
Bacteriophage T4
Temperature
Sorbitol
permeates
sorbitol
Sucrose
temperature
sucrose

ASJC Scopus subject areas

  • Immunology

Cite this

Stability and in vitro DNA packaging of bacteriophages : Effects of dextrans, sugars, and polyols. / Serwer, Philip; Masker, W. E.; Allen, J. L.

In: Journal of Virology, Vol. 45, No. 2, 1983, p. 665-671.

Research output: Contribution to journalArticle

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