Spread of artemisinin-resistant Plasmodium falciparum in Myanmar

A cross-sectional survey of the K13 molecular marker

Kyaw M. Tun, Mallika Imwong, Khin M. Lwin, Aye A. Win, Tin M. Hlaing, Thaung Hlaing, Khin Lin, Myat P. Kyaw, Katherine Plewes, M. Abul Faiz, Mehul Dhorda, Phaik Yeong Cheah, Sasithon Pukrittayakamee, Elizabeth A. Ashley, Tim J.C. Anderson, Shalini Nair, Marina McDew-White, Jennifer A. Flegg, Eric P.M. Grist, Philippe Guerin & 7 others Richard J. Maude, Frank Smithuis, Arjen M. Dondorp, Nicholas P.J. Day, François Nosten, Nicholas J. White, Charles J. Woodrow

Research output: Contribution to journalArticle

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Abstract

Background: Emergence of artemisinin resistance in southeast Asia poses a serious threat to the global control of Plasmodium falciparum malaria. Discovery of the K13 marker has transformed approaches to the monitoring of artemisinin resistance, allowing introduction of molecular surveillance in remote areas through analysis of DNA. We aimed to assess the spread of artemisinin-resistant P falciparum in Myanmar by determining the relative prevalence of P falciparum parasites carrying K13-propeller mutations. Methods: We did this cross-sectional survey at malaria treatment centres at 55 sites in ten administrative regions in Myanmar, and in relevant border regions in Thailand and Bangladesh, between January, 2013, and September, 2014. K13 sequences from P falciparum infections were obtained mainly by passive case detection. We entered data into two geostatistical models to produce predictive maps of the estimated prevalence of mutations of the K13 propeller region across Myanmar. Findings: Overall, 371 (39%) of 940 samples carried a K13-propeller mutation. We recorded 26 different mutations, including nine mutations not described previously in southeast Asia. In seven (70%) of the ten administrative regions of Myanmar, the combined K13-mutation prevalence was more than 20%. Geospatial mapping showed that the overall prevalence of K13 mutations exceeded 10% in much of the east and north of the country. In Homalin, Sagaing Region, 25 km from the Indian border, 21 (47%) of 45 parasite samples carried K13-propeller mutations. Interpretation: Artemisinin resistance extends across much of Myanmar. We recorded P falciparum parasites carrying K13-propeller mutations at high prevalence next to the northwestern border with India. Appropriate therapeutic regimens should be tested urgently and implemented comprehensively if spread of artemisinin resistance to other regions is to be avoided. Funding: Wellcome Trust-Mahidol University-Oxford Tropical Medicine Research Programme and the Bill & Melinda Gates Foundation.

Original languageEnglish (US)
Pages (from-to)415-421
Number of pages7
JournalThe Lancet Infectious Diseases
Volume15
Issue number4
DOIs
StatePublished - Apr 1 2015
Externally publishedYes

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Myanmar
Plasmodium falciparum
Cross-Sectional Studies
Mutation
Southeastern Asia
Parasites
Tropical Medicine
artemisinine
Bangladesh
Falciparum Malaria
Thailand
Malaria
India

ASJC Scopus subject areas

  • Infectious Diseases

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Spread of artemisinin-resistant Plasmodium falciparum in Myanmar : A cross-sectional survey of the K13 molecular marker. / Tun, Kyaw M.; Imwong, Mallika; Lwin, Khin M.; Win, Aye A.; Hlaing, Tin M.; Hlaing, Thaung; Lin, Khin; Kyaw, Myat P.; Plewes, Katherine; Faiz, M. Abul; Dhorda, Mehul; Cheah, Phaik Yeong; Pukrittayakamee, Sasithon; Ashley, Elizabeth A.; Anderson, Tim J.C.; Nair, Shalini; McDew-White, Marina; Flegg, Jennifer A.; Grist, Eric P.M.; Guerin, Philippe; Maude, Richard J.; Smithuis, Frank; Dondorp, Arjen M.; Day, Nicholas P.J.; Nosten, François; White, Nicholas J.; Woodrow, Charles J.

In: The Lancet Infectious Diseases, Vol. 15, No. 4, 01.04.2015, p. 415-421.

Research output: Contribution to journalArticle

Tun, KM, Imwong, M, Lwin, KM, Win, AA, Hlaing, TM, Hlaing, T, Lin, K, Kyaw, MP, Plewes, K, Faiz, MA, Dhorda, M, Cheah, PY, Pukrittayakamee, S, Ashley, EA, Anderson, TJC, Nair, S, McDew-White, M, Flegg, JA, Grist, EPM, Guerin, P, Maude, RJ, Smithuis, F, Dondorp, AM, Day, NPJ, Nosten, F, White, NJ & Woodrow, CJ 2015, 'Spread of artemisinin-resistant Plasmodium falciparum in Myanmar: A cross-sectional survey of the K13 molecular marker', The Lancet Infectious Diseases, vol. 15, no. 4, pp. 415-421. https://doi.org/10.1016/S1473-3099(15)70032-0
Tun, Kyaw M. ; Imwong, Mallika ; Lwin, Khin M. ; Win, Aye A. ; Hlaing, Tin M. ; Hlaing, Thaung ; Lin, Khin ; Kyaw, Myat P. ; Plewes, Katherine ; Faiz, M. Abul ; Dhorda, Mehul ; Cheah, Phaik Yeong ; Pukrittayakamee, Sasithon ; Ashley, Elizabeth A. ; Anderson, Tim J.C. ; Nair, Shalini ; McDew-White, Marina ; Flegg, Jennifer A. ; Grist, Eric P.M. ; Guerin, Philippe ; Maude, Richard J. ; Smithuis, Frank ; Dondorp, Arjen M. ; Day, Nicholas P.J. ; Nosten, François ; White, Nicholas J. ; Woodrow, Charles J. / Spread of artemisinin-resistant Plasmodium falciparum in Myanmar : A cross-sectional survey of the K13 molecular marker. In: The Lancet Infectious Diseases. 2015 ; Vol. 15, No. 4. pp. 415-421.
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abstract = "Background: Emergence of artemisinin resistance in southeast Asia poses a serious threat to the global control of Plasmodium falciparum malaria. Discovery of the K13 marker has transformed approaches to the monitoring of artemisinin resistance, allowing introduction of molecular surveillance in remote areas through analysis of DNA. We aimed to assess the spread of artemisinin-resistant P falciparum in Myanmar by determining the relative prevalence of P falciparum parasites carrying K13-propeller mutations. Methods: We did this cross-sectional survey at malaria treatment centres at 55 sites in ten administrative regions in Myanmar, and in relevant border regions in Thailand and Bangladesh, between January, 2013, and September, 2014. K13 sequences from P falciparum infections were obtained mainly by passive case detection. We entered data into two geostatistical models to produce predictive maps of the estimated prevalence of mutations of the K13 propeller region across Myanmar. Findings: Overall, 371 (39{\%}) of 940 samples carried a K13-propeller mutation. We recorded 26 different mutations, including nine mutations not described previously in southeast Asia. In seven (70{\%}) of the ten administrative regions of Myanmar, the combined K13-mutation prevalence was more than 20{\%}. Geospatial mapping showed that the overall prevalence of K13 mutations exceeded 10{\%} in much of the east and north of the country. In Homalin, Sagaing Region, 25 km from the Indian border, 21 (47{\%}) of 45 parasite samples carried K13-propeller mutations. Interpretation: Artemisinin resistance extends across much of Myanmar. We recorded P falciparum parasites carrying K13-propeller mutations at high prevalence next to the northwestern border with India. Appropriate therapeutic regimens should be tested urgently and implemented comprehensively if spread of artemisinin resistance to other regions is to be avoided. Funding: Wellcome Trust-Mahidol University-Oxford Tropical Medicine Research Programme and the Bill & Melinda Gates Foundation.",
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T1 - Spread of artemisinin-resistant Plasmodium falciparum in Myanmar

T2 - A cross-sectional survey of the K13 molecular marker

AU - Tun, Kyaw M.

AU - Imwong, Mallika

AU - Lwin, Khin M.

AU - Win, Aye A.

AU - Hlaing, Tin M.

AU - Hlaing, Thaung

AU - Lin, Khin

AU - Kyaw, Myat P.

AU - Plewes, Katherine

AU - Faiz, M. Abul

AU - Dhorda, Mehul

AU - Cheah, Phaik Yeong

AU - Pukrittayakamee, Sasithon

AU - Ashley, Elizabeth A.

AU - Anderson, Tim J.C.

AU - Nair, Shalini

AU - McDew-White, Marina

AU - Flegg, Jennifer A.

AU - Grist, Eric P.M.

AU - Guerin, Philippe

AU - Maude, Richard J.

AU - Smithuis, Frank

AU - Dondorp, Arjen M.

AU - Day, Nicholas P.J.

AU - Nosten, François

AU - White, Nicholas J.

AU - Woodrow, Charles J.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Background: Emergence of artemisinin resistance in southeast Asia poses a serious threat to the global control of Plasmodium falciparum malaria. Discovery of the K13 marker has transformed approaches to the monitoring of artemisinin resistance, allowing introduction of molecular surveillance in remote areas through analysis of DNA. We aimed to assess the spread of artemisinin-resistant P falciparum in Myanmar by determining the relative prevalence of P falciparum parasites carrying K13-propeller mutations. Methods: We did this cross-sectional survey at malaria treatment centres at 55 sites in ten administrative regions in Myanmar, and in relevant border regions in Thailand and Bangladesh, between January, 2013, and September, 2014. K13 sequences from P falciparum infections were obtained mainly by passive case detection. We entered data into two geostatistical models to produce predictive maps of the estimated prevalence of mutations of the K13 propeller region across Myanmar. Findings: Overall, 371 (39%) of 940 samples carried a K13-propeller mutation. We recorded 26 different mutations, including nine mutations not described previously in southeast Asia. In seven (70%) of the ten administrative regions of Myanmar, the combined K13-mutation prevalence was more than 20%. Geospatial mapping showed that the overall prevalence of K13 mutations exceeded 10% in much of the east and north of the country. In Homalin, Sagaing Region, 25 km from the Indian border, 21 (47%) of 45 parasite samples carried K13-propeller mutations. Interpretation: Artemisinin resistance extends across much of Myanmar. We recorded P falciparum parasites carrying K13-propeller mutations at high prevalence next to the northwestern border with India. Appropriate therapeutic regimens should be tested urgently and implemented comprehensively if spread of artemisinin resistance to other regions is to be avoided. Funding: Wellcome Trust-Mahidol University-Oxford Tropical Medicine Research Programme and the Bill & Melinda Gates Foundation.

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