Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments

J. Brooks Crickard, Kyle Kaniecki, Youngho Kwon, Patrick Sung, Eric C. Greene

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

During meiosis, the twoDNArecombinases Rad51 and Dmc1 form specialized presynaptic filaments that are adapted for performing recombination between homologous chromosomes. There is currently a limited understanding of how these two recombinases are organized within the meiotic presynaptic filament. Here, we used single molecule imaging to examine the properties of presynaptic complexes composed of both Rad51 and Dmc1. We demonstrate that Rad51 and Dmc1 have an intrinsic ability to self-segregate, even in the absence of any other recombination accessory proteins. Moreover, we found that the presence of Dmc1 stabilizes the adjacent Rad51 filaments, suggesting that cross-Talk between these two recombinases may affect their biochemical properties. Based upon these findings, we describe a model for the organization of Rad51 and Dmc1within the meiotic presynaptic complex,whichis also consistent with in vivo observations, genetic findings, and biochemical expectations. This model argues against the existence of extensively intermixed filaments, and we propose that Rad51 and Dmc1 have intrinsic capacities to form spatially distinct filaments, suggesting that additional recombination cofactors are not required to segregate the Rad51 and Dmc1 filaments.

Original languageEnglish (US)
Pages (from-to)4191-4200
Number of pages10
JournalJournal of Biological Chemistry
Volume293
Issue number11
DOIs
StatePublished - Mar 16 2018
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Spontaneous self-segregation of Rad51 and Dmc1 DNA recombinases within mixed recombinase filaments'. Together they form a unique fingerprint.

  • Cite this