TY - JOUR
T1 - Splenectomy differentially influences immune responses in various tissue compartments of the body
AU - Shih-Ching, Kang
AU - Choudhry, Mashkoor A.
AU - Matsutani, Takeshi
AU - Schwacha, Martin G.
AU - Rue, Loring W.
AU - Bland, Kirby I.
AU - Chaudry, Irshad H.
N1 - Funding Information:
We would like to thank Ms. Shellie Hyde and Dr. Shadab Rana for their excellent technical assistance in this study. This work was supported by NIH Grant RO1 GM37127.
PY - 2004/11/7
Y1 - 2004/11/7
N2 - Patients without spleens have an increased risk of infection. Previous studies have shown that splenectomy (Spx) influences Kupffer cells (KC), peritoneal macrophages (pMφ) and alveolar macrophages (aMφ) phagocytosis and bactericidal activity. This study examined the effect of Spx on the cytokine production by peripheral blood monocular cells (PBMC), KC, aMφ, pMφ and cells from mesenteric lymph nodes (MLN). We also determined if Spx influences survival following sepsis induced by cecal ligation and puncture (CLP). C57BL/6J male mice (8-10 weeks old) underwent sham operation or Spx. Two weeks after sham or Spx, KC, pMφ, aMφ, PBMC and cells from MLN were isolated and stimulated with LPS. Cell-free supernatants were analyzed for TNF-α, IL-6, and IL-10. A significant increase in KC, pMφ and aMφ TNF-α and IL-6 release was observed following Spx. The production of IL-10 was also significantly higher in KC under those conditions. In contrast, the release of TNF-α, IL-6 and IL-10 was significantly decreased in PBMC after Spx. Similarly, TNF-α and IL-6 was also decreased in MLN after Spx. Overall survival after CLP was not different in mice subjected to either sham or Spx. However, the mean time to death was significantly decreased in mice subjected to Spx compared to sham injured mice. These findings suggest that Spx modulates immune cell functions in various tissue compartments of the body and results in early deaths following sepsis.
AB - Patients without spleens have an increased risk of infection. Previous studies have shown that splenectomy (Spx) influences Kupffer cells (KC), peritoneal macrophages (pMφ) and alveolar macrophages (aMφ) phagocytosis and bactericidal activity. This study examined the effect of Spx on the cytokine production by peripheral blood monocular cells (PBMC), KC, aMφ, pMφ and cells from mesenteric lymph nodes (MLN). We also determined if Spx influences survival following sepsis induced by cecal ligation and puncture (CLP). C57BL/6J male mice (8-10 weeks old) underwent sham operation or Spx. Two weeks after sham or Spx, KC, pMφ, aMφ, PBMC and cells from MLN were isolated and stimulated with LPS. Cell-free supernatants were analyzed for TNF-α, IL-6, and IL-10. A significant increase in KC, pMφ and aMφ TNF-α and IL-6 release was observed following Spx. The production of IL-10 was also significantly higher in KC under those conditions. In contrast, the release of TNF-α, IL-6 and IL-10 was significantly decreased in PBMC after Spx. Similarly, TNF-α and IL-6 was also decreased in MLN after Spx. Overall survival after CLP was not different in mice subjected to either sham or Spx. However, the mean time to death was significantly decreased in mice subjected to Spx compared to sham injured mice. These findings suggest that Spx modulates immune cell functions in various tissue compartments of the body and results in early deaths following sepsis.
KW - CLP
KW - Cytokines
KW - Kupffer cell
KW - Macrophage
KW - Mesenteric lymph node
KW - PBMC
UR - http://www.scopus.com/inward/record.url?scp=4744373317&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4744373317&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2004.07.005
DO - 10.1016/j.cyto.2004.07.005
M3 - Article
C2 - 15473952
AN - SCOPUS:4744373317
SN - 1043-4666
VL - 28
SP - 101
EP - 108
JO - Cytokine
JF - Cytokine
IS - 3
ER -