TY - JOUR
T1 - Spinal cord cultured neurons
T2 - an in vitro model to study GABA synaptic pharmacology.
AU - Ticku, M.
AU - Mehta, A.
AU - Lehoullier, P.
PY - 1988
Y1 - 1988
N2 - In summary, spinal cord cultured neurons provide an in vitro model for studying GABA synaptic pharmacology. GABAA agonists stimulate 36Cl-influx in these cells in a concentration-dependent manner. The enhancing effect of GABA is potentiated by BZ agonists, inhibited by GABA antagonists, and attenuated by the inverse agonist beta-carboline, DMCM. These cells also exhibit the specific binding of [3H]flunitrazepam with a pharmacological specificity of the central BZ receptors. Finally, BZ receptors are coupled to GABA, barbiturate, and picrotoxin sites in these cells, as indicated by allosteric interactions both by binding and 36Cl-influx assay.
AB - In summary, spinal cord cultured neurons provide an in vitro model for studying GABA synaptic pharmacology. GABAA agonists stimulate 36Cl-influx in these cells in a concentration-dependent manner. The enhancing effect of GABA is potentiated by BZ agonists, inhibited by GABA antagonists, and attenuated by the inverse agonist beta-carboline, DMCM. These cells also exhibit the specific binding of [3H]flunitrazepam with a pharmacological specificity of the central BZ receptors. Finally, BZ receptors are coupled to GABA, barbiturate, and picrotoxin sites in these cells, as indicated by allosteric interactions both by binding and 36Cl-influx assay.
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M3 - Article
C2 - 2845734
AN - SCOPUS:0023721164
VL - 45
SP - 151
EP - 159
JO - Advances in biochemical psychopharmacology
JF - Advances in biochemical psychopharmacology
SN - 0065-2229
ER -