Sphingolipid biosynthetic pathway genes FEN1 and SUR4 modulate amphotericin B resistance

Sushma Sharma, Md Alfatah, Vinay K. Bari, Yashpal Rawal, Sanjoy Paul, K. Ganesan

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Deletants of the sphingolipid biosynthetic pathway genes FEN1 and SUR4 of Saccharomyces cerevisiae, as well as deletants of their orthologs in Candida albicans, were found to be 2- to 5-fold-more sensitive to amphotericin B (AmB) than parent strains. The inhibition of sphingolipid biosynthesis in parent strains by myriocin sensitized them to AmB, which can be reversed by providing phytosphingosine, an intermediate in the sphingolipid pathway. These results indicate that sphingolipids modulate AmB resistance, with implications for mechanisms underlying AmB action and resistance.

Original languageEnglish (US)
Pages (from-to)2409-2414
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume58
Issue number4
DOIs
StatePublished - Apr 2014
Externally publishedYes

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Fingerprint

Dive into the research topics of 'Sphingolipid biosynthetic pathway genes FEN1 and SUR4 modulate amphotericin B resistance'. Together they form a unique fingerprint.

Cite this