Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study

Gihan M. Bareh; Ethan Jacoby; Peter Binkley; Tien cheng Chang; Robert S. Schenken; Randal D. Robinson

doi:10.1016/j.fertnstert.2015.10.033

Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study

Gihan M. Bareh, Ethan Jacoby, Peter Binkley, Tien cheng Chang, Robert S. Schenken, Randal D. Robinson

Department of Obstetrics & Gynecology

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

Objective To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL). Design Prospective, cohort study. Setting Academic tertiary care center. Patient(s) Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility. Intervention(s) Semen samples were collected by masturbation after 48–72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with >20 × 10⁶ spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis. Main Outcome Measure(s) Sperm DNA fragmentation in both groups. Result(s) Mean DNA fragmentation (mean ± SD) was significantly more in men with RPL (36.8 ± 5) compared with controls (9.4 ± 2.7). Conclusion(s) Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

Original language	English (US)
Pages (from-to)	329-336.e1
Journal	Fertility and sterility
Volume	105
Issue number	2
DOIs	https://doi.org/10.1016/j.fertnstert.2015.10.033
State	Published - Feb 1 2016

Keywords

Sperm DNA
TUNEL assay
recurrent pregnancy loss
unexplained infertility

ASJC Scopus subject areas

Reproductive Medicine
Obstetrics and Gynecology

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10.1016/j.fertnstert.2015.10.033

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title = "Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study",

abstract = "Objective To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL). Design Prospective, cohort study. Setting Academic tertiary care center. Patient(s) Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility. Intervention(s) Semen samples were collected by masturbation after 48–72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with >20 × 106 spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis. Main Outcome Measure(s) Sperm DNA fragmentation in both groups. Result(s) Mean DNA fragmentation (mean ± SD) was significantly more in men with RPL (36.8 ± 5) compared with controls (9.4 ± 2.7). Conclusion(s) Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.",

keywords = "Sperm DNA, TUNEL assay, recurrent pregnancy loss, unexplained infertility",

author = "Bareh, {Gihan M.} and Ethan Jacoby and Peter Binkley and Chang, {Tien cheng} and Schenken, {Robert S.} and Robinson, {Randal D.}",

note = "Publisher Copyright: {\textcopyright} 2016 American Society for Reproductive Medicine",

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T2 - a prospective study

AU - Bareh, Gihan M.

AU - Jacoby, Ethan

AU - Binkley, Peter

AU - Chang, Tien cheng

AU - Schenken, Robert S.

AU - Robinson, Randal D.

PY - 2016/2/1

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N2 - Objective To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL). Design Prospective, cohort study. Setting Academic tertiary care center. Patient(s) Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility. Intervention(s) Semen samples were collected by masturbation after 48–72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with >20 × 106 spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis. Main Outcome Measure(s) Sperm DNA fragmentation in both groups. Result(s) Mean DNA fragmentation (mean ± SD) was significantly more in men with RPL (36.8 ± 5) compared with controls (9.4 ± 2.7). Conclusion(s) Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

AB - Objective To determine whether sperm DNA integrity in normozoospermic male partners plays a role in idiopathic recurrent pregnancy loss (RPL). Design Prospective, cohort study. Setting Academic tertiary care center. Patient(s) Group I: 26 male partners of women with unexplained RPL. Group II: 31 normozoospermic males with proven fertility. Intervention(s) Semen samples were collected by masturbation after 48–72 hours of abstinence. After liquefaction at room temperature, semen analysis was performed according to World Health Organization standards. Only samples with >20 × 106 spermatozoa/mL with at least 50% progressive sperm motility and 30 % normal morphology were selected for the study. DNA fragmentation of the sperm was assessed with TUNEL assay followed by flow cytometric analysis. Main Outcome Measure(s) Sperm DNA fragmentation in both groups. Result(s) Mean DNA fragmentation (mean ± SD) was significantly more in men with RPL (36.8 ± 5) compared with controls (9.4 ± 2.7). Conclusion(s) Sperm DNA fragmentation may play a role in unexplained RPL despite normal semen analysis parameters.

KW - Sperm DNA

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KW - unexplained infertility

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Sperm deoxyribonucleic acid fragmentation assessment in normozoospermic male partners of couples with unexplained recurrent pregnancy loss: a prospective study

Abstract

Keywords

ASJC Scopus subject areas

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