Specific localization in the equatorial region of gp20, a 20 kDa sialylglycoprotein of the capacitated human spermatozoon acquired during epididymal transit which is necessary to penetrate zona-free hamster eggs

Riccardo Focarelli, Andrea Giuffrida, Sonia Capparelli, Marcello Scibona, Fabrizio Menchini Fabris, Felice Francavilla, Sandro Francavilla, Cinzia Della Giovampaola, Floriana Rosati

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

In this study we set out to characterize gp20, a 20 kDa glycoprotein of the human sperm surface, first identified by us by radiolabelling the sialic acid residues of the sperm surface [R. Focarelli et al. (1995), Mol. Hum. Reprod., 2, 2755-2759). The protein was partially purified from pooled sperm samples of several healthy donors and used to raise a specific antiserum to study its localization in the reproductive system. When tested with freshly ejaculated spermatozoa, the anti-gp20 antibody intensely stained the head and midpiece. However, on capacitated spermatozoa the antigen was restricted to a sharp zone in the equatorial region. The antibody did not bind to differentiating germ cells but the antigen was present in epididymal epithelial cells and also in seminal plasma. Anti-gp20 exerted a blocking effect in a test for sperm penetration of zona-free hamster eggs, thus suggesting that gp20 is involved in the early stages of fertilization.

Original languageEnglish (US)
Pages (from-to)119-125
Number of pages7
JournalMolecular Human Reproduction
Volume4
Issue number2
DOIs
StatePublished - Feb 1998
Externally publishedYes

Fingerprint

Herpes Zoster
Cricetinae
Eggs
Spermatozoa
Sperm-Ovum Interactions
Antigens
N-Acetylneuraminic Acid
Semen
Fertilization
Germ Cells
Immune Sera
Anti-Idiotypic Antibodies
Glycoproteins
Epithelial Cells
Head
Antibodies
Proteins

Keywords

  • Antibodies
  • Capacitation
  • Epididymal maturation
  • Fertilization
  • Human spermatozoa

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Specific localization in the equatorial region of gp20, a 20 kDa sialylglycoprotein of the capacitated human spermatozoon acquired during epididymal transit which is necessary to penetrate zona-free hamster eggs. / Focarelli, Riccardo; Giuffrida, Andrea; Capparelli, Sonia; Scibona, Marcello; Fabris, Fabrizio Menchini; Francavilla, Felice; Francavilla, Sandro; Della Giovampaola, Cinzia; Rosati, Floriana.

In: Molecular Human Reproduction, Vol. 4, No. 2, 02.1998, p. 119-125.

Research output: Contribution to journalArticle

Focarelli, Riccardo ; Giuffrida, Andrea ; Capparelli, Sonia ; Scibona, Marcello ; Fabris, Fabrizio Menchini ; Francavilla, Felice ; Francavilla, Sandro ; Della Giovampaola, Cinzia ; Rosati, Floriana. / Specific localization in the equatorial region of gp20, a 20 kDa sialylglycoprotein of the capacitated human spermatozoon acquired during epididymal transit which is necessary to penetrate zona-free hamster eggs. In: Molecular Human Reproduction. 1998 ; Vol. 4, No. 2. pp. 119-125.
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