Specific and sensitive radioimmunoassay for 3-methoxy-4- hydroxyphenylethyleneglycol(MOPEG)

T. Kent Keeton, Henry Krutzsch, Walter Lovenberg

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Antibodies that specifically bind the norepinephrine metabolite 3-methoxy-4-hydroxyphenylethyleneglycol (MOPEG) were produced in rabbits after injection of a derivative of MOPEG conjugated with bovine thyroglobulin. A sensitive radioimmunoassay was devised with this antiserum, in which as little as 0.5 nanogram of MOPEG can be accurately measured with a final antibody dilution of 1:180. The antibody appears to be specific for MOPEG, since tritiated MOPEG was not displaced from the antibodies by norepinephrine, epinephrine, dopamine, serotonin, or their major metabolites, including MOPEG-sulfate (333 nanograms each).

Original languageEnglish (US)
Pages (from-to)586-588
Number of pages3
JournalScience
Volume211
Issue number4482
DOIs
StatePublished - 1981
Externally publishedYes

ASJC Scopus subject areas

  • General

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