Spatial regulation of DELTA expression mediates NOTCH signalling for segmentation of Drosophila legs

Arati Mishra, Namita Agrawal, Swati Banerjee, Dyuti Sardesai, Jasbir Singh Dalal, Jyoti Bhojwani, Pradip Sinha

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The Notch (N) signalling pathway is recruited for segregation of cell fates in a number of Drosophila tissue types. We show here that N dependent segmentation of Drosophila legs is regulated by a dynamic pattern of expression of its ligand, DELTA (DL). During third larval instar and early stages of pupation, high levels of DL expression is seen in stripes of cells in the leg imaginal discs which later form the proximal borders of leg joints. These domains also displayed heightened Dl enhancer activity. During subsequent stages of pupation, following segmentation of the leg primordium, DL expression becomes uniform throughout these segments barring the joints. We further show that regulatory Dl mutations or mis-expression of DL abolish leg segmentation. Domains of N signalling for segmentation of legs of flies are thus set up by a stringent spatial regulation of expression of its ligand at the segment border. Further, a comparable role of DL in antennal development reveals a common paradigm of DL-N signalling for segmentation of appendages in flies.

Original languageEnglish (US)
Pages (from-to)115-127
Number of pages13
JournalMechanisms of Development
Volume105
Issue number1-2
DOIs
Publication statusPublished - Jan 1 2001

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Keywords

  • Antenna
  • Delta
  • Drosophila
  • Leg
  • Notch
  • Segmentation

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Mishra, A., Agrawal, N., Banerjee, S., Sardesai, D., Dalal, J. S., Bhojwani, J., & Sinha, P. (2001). Spatial regulation of DELTA expression mediates NOTCH signalling for segmentation of Drosophila legs. Mechanisms of Development, 105(1-2), 115-127. https://doi.org/10.1016/S0925-4773(01)00387-2