Spatial refractive index measurement of porcine artery using differential phase OCT

Jeehyun Kim, Digant P. Dave, Chris Rylander, Marc Feldman, Sharon Thomsen, Thomas E. Milner

Research output: Contribution to journalConference articlepeer-review


We describe a methodology to record spatial variation of refractive index of porcine renal artery using differential phase optical coherence tomography (DP-OCT). DP-OCT provides a quantitative measure of thin specimen phase retardation and refractive index with phase resolution of 5 nm and lateral resolution of 3 μm. DP-OCT instrumentation is an all-fiber, dual channel Mchelson interferometer constructed using polarization maintaining fiber. Two orthogonal polarization modes of light are spatially separated using a Wollaston prism and directed into separate photoreceivers. Because phase noise in the environment is equally present in both channels, computation of phase difference between the two signal channels is attributed exclusively to variation in the specimen's composite refractive index. Porcine renal artery is freshly harvested from a local slaughter house. The lumen is cut open and sliced at 5 μm thickness. Microscopic slide for the tissue section is processed by standard histology method with mounting media. Two dimensional en face dual-channel phase images are taken over 150 μm ×200 μm region on the microscopic slide and the images are reconstructed by plotting relative phase variation as the OCT beam is moved across the artery cross section.

Original languageEnglish (US)
Pages (from-to)167-171
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Oct 27 2004
Externally publishedYes
EventProgress in Biomedical Optics and Imaging - Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine VIII - San Jose, CA, United States
Duration: Jan 26 2004Jan 28 2004


  • Phase sensitive OCT
  • Refractive index
  • Relative phase retardation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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