Spatial cognitive deficits in an animal model of Wernicke-Korsakoff syndrome are related to changes in thalamic VDAC protein concentrations

K. O. Bueno; L. de Souza Resende; A. F. Ribeiro; D. M. dos Santos; E. C. Gonçalves; F. A.B. Vigil; I. F. de Oliveira Silva; L. F. Ferreira; A. M. de Castro Pimenta; A. M. Ribeiro

doi:10.1016/j.neuroscience.2015.03.001

Spatial cognitive deficits in an animal model of Wernicke-Korsakoff syndrome are related to changes in thalamic VDAC protein concentrations

K. O. Bueno, L. de Souza Resende, A. F. Ribeiro, D. M. dos Santos, E. C. Gonçalves, F. A.B. Vigil, I. F. de Oliveira Silva, L. F. Ferreira, A. M. de Castro Pimenta, A. M. Ribeiro

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11 Scopus citations

Abstract

Proteomic profiles of the thalamus and the correlation between the rats' performance on a spatial learning task and differential protein expression were assessed in the thiamine deficiency (TD) rat model of Wernicke-Korsakoff syndrome. Two-dimensional gel-electrophoresis detected 320 spots and a significant increase or decrease in seven proteins. Four proteins were correlated to rat behavioral performance in the Morris Water Maze. One of the four proteins was identified by mass spectrometry as Voltage-Dependent Anion Channels (VDACs). The association of VDAC is evident in trials in which the rats' performance was worst, in which the VDAC protein was reduced, as confirmed by Western blot. No difference was observed on the mRNA of Vdac genes, indicating that the decreased VDAC expression may be related to a post-transcriptional process. The results show that TD neurodegeneration involves changes in thalamic proteins and suggest that VDAC protein activity might play an important role in an initial stage of the spatial learning process.

Original language	English (US)
Pages (from-to)	29-37
Number of pages	9
Journal	Neuroscience
Volume	294
DOIs	https://doi.org/10.1016/j.neuroscience.2015.03.001
State	Published - May 1 2015
Externally published	Yes

Keywords

Proteomic
Rats
Spatial learning
Thalamus
Thiamine deficiency
VDAC

ASJC Scopus subject areas

General Neuroscience

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10.1016/j.neuroscience.2015.03.001

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Bueno, K. O., de Souza Resende, L., Ribeiro, A. F., dos Santos, D. M., Gonçalves, E. C., Vigil, F. A. B., de Oliveira Silva, I. F., Ferreira, L. F., de Castro Pimenta, A. M., & Ribeiro, A. M. (2015). Spatial cognitive deficits in an animal model of Wernicke-Korsakoff syndrome are related to changes in thalamic VDAC protein concentrations. Neuroscience, 294, 29-37. https://doi.org/10.1016/j.neuroscience.2015.03.001

Bueno, KO, de Souza Resende, L, Ribeiro, AF, dos Santos, DM, Gonçalves, EC, Vigil, FAB, de Oliveira Silva, IF, Ferreira, LF, de Castro Pimenta, AM & Ribeiro, AM 2015, 'Spatial cognitive deficits in an animal model of Wernicke-Korsakoff syndrome are related to changes in thalamic VDAC protein concentrations', Neuroscience, vol. 294, pp. 29-37. https://doi.org/10.1016/j.neuroscience.2015.03.001

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title = "Spatial cognitive deficits in an animal model of Wernicke-Korsakoff syndrome are related to changes in thalamic VDAC protein concentrations",

abstract = "Proteomic profiles of the thalamus and the correlation between the rats' performance on a spatial learning task and differential protein expression were assessed in the thiamine deficiency (TD) rat model of Wernicke-Korsakoff syndrome. Two-dimensional gel-electrophoresis detected 320 spots and a significant increase or decrease in seven proteins. Four proteins were correlated to rat behavioral performance in the Morris Water Maze. One of the four proteins was identified by mass spectrometry as Voltage-Dependent Anion Channels (VDACs). The association of VDAC is evident in trials in which the rats' performance was worst, in which the VDAC protein was reduced, as confirmed by Western blot. No difference was observed on the mRNA of Vdac genes, indicating that the decreased VDAC expression may be related to a post-transcriptional process. The results show that TD neurodegeneration involves changes in thalamic proteins and suggest that VDAC protein activity might play an important role in an initial stage of the spatial learning process.",

keywords = "Proteomic, Rats, Spatial learning, Thalamus, Thiamine deficiency, VDAC",

author = "Bueno, {K. O.} and {de Souza Resende}, L. and Ribeiro, {A. F.} and {dos Santos}, {D. M.} and Gon{\c c}alves, {E. C.} and Vigil, {F. A.B.} and {de Oliveira Silva}, {I. F.} and Ferreira, {L. F.} and {de Castro Pimenta}, {A. M.} and Ribeiro, {A. M.}",

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AU - de Souza Resende, L.

AU - Ribeiro, A. F.

AU - dos Santos, D. M.

AU - Gonçalves, E. C.

AU - Vigil, F. A.B.

AU - de Oliveira Silva, I. F.

AU - Ferreira, L. F.

AU - de Castro Pimenta, A. M.

AU - Ribeiro, A. M.

PY - 2015/5/1

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N2 - Proteomic profiles of the thalamus and the correlation between the rats' performance on a spatial learning task and differential protein expression were assessed in the thiamine deficiency (TD) rat model of Wernicke-Korsakoff syndrome. Two-dimensional gel-electrophoresis detected 320 spots and a significant increase or decrease in seven proteins. Four proteins were correlated to rat behavioral performance in the Morris Water Maze. One of the four proteins was identified by mass spectrometry as Voltage-Dependent Anion Channels (VDACs). The association of VDAC is evident in trials in which the rats' performance was worst, in which the VDAC protein was reduced, as confirmed by Western blot. No difference was observed on the mRNA of Vdac genes, indicating that the decreased VDAC expression may be related to a post-transcriptional process. The results show that TD neurodegeneration involves changes in thalamic proteins and suggest that VDAC protein activity might play an important role in an initial stage of the spatial learning process.

AB - Proteomic profiles of the thalamus and the correlation between the rats' performance on a spatial learning task and differential protein expression were assessed in the thiamine deficiency (TD) rat model of Wernicke-Korsakoff syndrome. Two-dimensional gel-electrophoresis detected 320 spots and a significant increase or decrease in seven proteins. Four proteins were correlated to rat behavioral performance in the Morris Water Maze. One of the four proteins was identified by mass spectrometry as Voltage-Dependent Anion Channels (VDACs). The association of VDAC is evident in trials in which the rats' performance was worst, in which the VDAC protein was reduced, as confirmed by Western blot. No difference was observed on the mRNA of Vdac genes, indicating that the decreased VDAC expression may be related to a post-transcriptional process. The results show that TD neurodegeneration involves changes in thalamic proteins and suggest that VDAC protein activity might play an important role in an initial stage of the spatial learning process.

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KW - Rats

KW - Spatial learning

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KW - Thiamine deficiency

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Spatial cognitive deficits in an animal model of Wernicke-Korsakoff syndrome are related to changes in thalamic VDAC protein concentrations

Abstract

Keywords

ASJC Scopus subject areas

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