Spatial approximation between a photolabile residue in position 13 of secretin and the amino terminus of the secretin receptor

Mengwei Zang, Maoqing Dong, Delia I. Pinon, Xi Qin Ding, Elizabeth M. Hadac, Zhijun Li, Terry P. Lybrand, Laurence J. Miller

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The amino-terminal domain of class B G protein-coupled receptors is critically important for natural peptide agonist binding and action. The precise role it plays and the molecular basis of the interaction between ligand and this domain are not well understood. In the current work, we have developed a new probe for affinity labeling the secretin receptor through a photolabile benzoyl-phenylalanine residue in position 13. This represented a high affinity ligand (Ki = 56 ± 8 nM) that was a potent full agonist to stimulate cellular cAMP (EC50 = 236 ± 22 pM). It covalently labeled the secretin receptor saturably in a single site. This was localized to the amino-terminal domain near the first transmembrane segment using a series of chemical and enzymatic digestions. Edman degradation sequencing of radiolabeled cyanogen bromide and skatole digestion products that were attached to glass beads and further cleaved with endoproteinase Asp-N demonstrated that the labeled residue represented Val103. This is in contrast with previous photoaffinity labeling through positions 6, 18, 22, and 26 of secretin that all labeled the distal end of the amino terminus of this receptor. Together, these five pairs of residue-residue approximations provide important constraints to better understand the molecular conformation of the agonist-bound receptor.

Original languageEnglish (US)
Pages (from-to)993-1001
Number of pages9
JournalMolecular Pharmacology
Volume63
Issue number5
DOIs
StatePublished - May 1 2003
Externally publishedYes

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Secretin
endoproteinase Asp-N
Digestion
Skatole
Ligands
Molecular Conformation
Cyanogen Bromide
G-Protein-Coupled Receptors
Phenylalanine
Glass
Peptides
secretin receptor

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Medicine
  • Pharmacology

Cite this

Spatial approximation between a photolabile residue in position 13 of secretin and the amino terminus of the secretin receptor. / Zang, Mengwei; Dong, Maoqing; Pinon, Delia I.; Ding, Xi Qin; Hadac, Elizabeth M.; Li, Zhijun; Lybrand, Terry P.; Miller, Laurence J.

In: Molecular Pharmacology, Vol. 63, No. 5, 01.05.2003, p. 993-1001.

Research output: Contribution to journalArticle

Zang, Mengwei ; Dong, Maoqing ; Pinon, Delia I. ; Ding, Xi Qin ; Hadac, Elizabeth M. ; Li, Zhijun ; Lybrand, Terry P. ; Miller, Laurence J. / Spatial approximation between a photolabile residue in position 13 of secretin and the amino terminus of the secretin receptor. In: Molecular Pharmacology. 2003 ; Vol. 63, No. 5. pp. 993-1001.
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