Solution structure and backbone dynamics of the TGFβ type II receptor extracellular domain

Shashank Deep, Kerfoot P. Walker, Zhanyong Shu, Andrew P. Hinck

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Isoforms of transforming growth factor β (TGFβ) are 25 kDa homodimeric polypeptides that signal by binding and bringing together two related, functionally distinct cell surface receptors designated as TβR1 and TβR2. Here, we report the solution structure of the 13.8 kDa extracellular domain of human TβR2 (ecTβR2) as calculated from N N-HN, Cα-Hα, and Cα-CO residual dipolar coupling restraints in conjunction with NOE distance, dihedral angle, and scalar coupling restraints. Comparison of the free ecTβR2 solution structure with the TGFβ3-bound ecTβR2 crystal structure reveals backbone conformations that superimpose with RMSDs of 1.0 Å over the regions of regular secondary structure and 1.4 Å overall. The differences in structure fall mainly in loop regions that are either poorly defined by the available NMR data or are involved in crystal contacts. The noted similarities between the NMR structure of the free form and the crystal structure of the TGFβ-bound form are also consistent with the close correspondence, 0.16 Å RMSD for regions of secondary structure and 0.51 Å RMSD overall, for the crystal structure of free ecTβR2 as compared to the crystal structure of TGFβ3-bound ecTβR2. Despite the apparent similarities between the free and the bound forms, there appears to be small but significant differences in structure involving the interfacial contact region of the receptor. Measurements of backbone 15N relaxation times and interpretation of these by the model-free formalism with axial diffusional anisotropy further reveal significant ms to μs time scale motions centered about two of the conserved disulfide bonds and in several residues that comprise the TGFβ binding surface. Together, these observations indicate that binding likely occurs through a mechanism with a small component of induced fit character, whereby flexibility within the receptor facilitates the transition to the TGFβ-bound state.

Original languageEnglish (US)
Pages (from-to)10126-10139
Number of pages14
JournalBiochemistry
Volume42
Issue number34
DOIs
StatePublished - Sep 2 2003

ASJC Scopus subject areas

  • Biochemistry

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