Soluble FLT1 Gene Therapy Alleviates Brain Arteriovenous Malformation Severity

Wan Zhu, Fanxia Shen, Lei Mao, Lei Zhan, Shuai Kang, Zhengda Sun, Jeffrey Nelson, Rui Zhang, Dingquan Zou, Cameron M. McDougall, Michael T. Lawton, Thiennu H. Vu, Zhijian Wu, Abraham Scaria, Peter Colosi, John Forsayeth, Hua Su

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8 Scopus citations

Abstract

Background and Purpose - Brain arteriovenous malformation (bAVM) is an important risk factor for intracranial hemorrhage. Current therapies are associated with high morbidities. Excessive vascular endothelial growth factor has been implicated in bAVM pathophysiology. Because soluble FLT1 binds to vascular endothelial growth factor with high affinity, we tested intravenous delivery of an adeno-associated viral vector serotype-9 expressing soluble FLT1 (AAV9-sFLT1) to alleviate the bAVM phenotype. Methods - Two mouse models were used. In model 1, bAVM was induced in R26CreER;Eng2f/2f mice through global Eng gene deletion and brain focal angiogenic stimulation; AAV2-sFLT02 (an AAV expressing a shorter form of sFLT1) was injected into the brain at the time of model induction, and AAV9-sFLT1, intravenously injected 8 weeks after. In model 2, SM22αCre;Eng2f/2f mice had a 90% occurrence of spontaneous bAVM at 5 weeks of age and 50% mortality at 6 weeks; AAV9-sFLT1 was intravenously delivered into 4- to 5-week-old mice. Tissue samples were collected 4 weeks after AAV9-sFLT1 delivery. Results - AAV2-sFLT02 inhibited bAVM formation, and AAV9-sFLT1 reduced abnormal vessels in model 1 (GFP versus sFLT1: 3.66±1.58/200 vessels versus 1.98±1.29, P<0.05). AAV9-sFLT1 reduced the occurrence of bAVM (GFP versus sFLT1: 100% versus 36%) and mortality (GFP versus sFLT1: 57% [12/22 mice] versus 24% [4/19 mice], P<0.05) in model 2. Kidney and liver function did not change significantly. Minor liver inflammation was found in 56% of AAV9-sFLT1-treated model 1 mice. Conclusions - By applying a regulated mechanism to restrict sFLT1 expression to bAVM, AAV9-sFLT1 can potentially be developed into a safer therapy to reduce the bAVM severity.

Original languageEnglish (US)
Pages (from-to)1420-1423
Number of pages4
JournalStroke
Volume48
Issue number5
DOIs
StatePublished - May 1 2017

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Keywords

  • angiogenesis
  • animals
  • brain
  • gene therapy

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Cite this

Zhu, W., Shen, F., Mao, L., Zhan, L., Kang, S., Sun, Z., Nelson, J., Zhang, R., Zou, D., McDougall, C. M., Lawton, M. T., Vu, T. H., Wu, Z., Scaria, A., Colosi, P., Forsayeth, J., & Su, H. (2017). Soluble FLT1 Gene Therapy Alleviates Brain Arteriovenous Malformation Severity. Stroke, 48(5), 1420-1423. https://doi.org/10.1161/STROKEAHA.116.015713