Sodium saccharin inhibits adenylyl cyclase activity in non-taste cells

Karim Dib, Francine Wrisez, Amina El Jamali, Bernard Lambert, Claude Correze

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Abstract

We have studied the in vitro effect of sodium saccharin (NaSacch) on the rat adipocyte adenylyl cyclase complex. NaSacch (2.5-50 mM) inhibited significantly in a dose-dependent manner basal and isoproterenol-stimulated cAMP accumulation on isolated rat adipocytes. Similarly, NaSacch (2.5-50 mM) inhibited forskolin-stimulated adenylyl cyclase activity measured in the presence of Mg2+-ATP on adipocyte, astrocyte and thyrocyte membrane fractions. In contrast, NaSacch did not inhibit but slightly increased the forskolin-stimulated adenylyl cyclase activity measured in the presence of Mn2+-ATP and GDPβS, a stable GDP analogue. The effect of NaSacch was not mediated through either the A1-adenosine receptor (A1R) or the α2-adrenergic receptor (α2AR). The inhibitory effect of NaSacch was additive to that of A1R agonist and was not blocked by the addition of the α2AR antagonist RX 821002. Pretreatment of adipocytes with pertussis toxin slightly attenuated but did not abolish the inhibitory effect of NaSacch on forskolin-stimulated-adenylyl cyclase activity on membrane fractions. These data suggest that the inhibitory effect of NaSacch on forskolin stimulated-adenylyl cyclase in adipocytes does not imply only Gi protein but also other direct or indirect inhibitory pathway(s) which remain to be determined.

Original languageEnglish (US)
Pages (from-to)431-438
Number of pages8
JournalCellular Signalling
Volume9
Issue number6
DOIs
StatePublished - Sep 1 1997

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Keywords

  • A-adenosine receptor
  • Adenylyl cyclase
  • Adipocytes
  • G-proteins
  • Pertussis toxin
  • Sodium saccharin
  • α-adrenergic receptor

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dib, K., Wrisez, F., El Jamali, A., Lambert, B., & Correze, C. (1997). Sodium saccharin inhibits adenylyl cyclase activity in non-taste cells. Cellular Signalling, 9(6), 431-438. https://doi.org/10.1016/S0898-6568(97)00033-8