Sodium independence of the binding of [3H]GBR 12783 and other dopamine uptake inhibitors to the dopamine uptake complex

Saloua Benmansour, Jean Jacques Bonnet, Philippe Protais, Jean Costentin

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When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.

Original languageEnglish (US)
Pages (from-to)97-102
Number of pages6
JournalNeuroscience Letters
Issue number1
StatePublished - Jun 1 1987



  • Binding in vitro
  • Catecholamine uptake inhibitor
  • Dopamine uptake system
  • Na-dependence

ASJC Scopus subject areas

  • Neuroscience(all)

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