Abstract
When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.
Original language | English (US) |
---|---|
Pages (from-to) | 97-102 |
Number of pages | 6 |
Journal | Neuroscience Letters |
Volume | 77 |
Issue number | 1 |
DOIs | |
State | Published - Jun 1 1987 |
Keywords
- Binding in vitro
- Catecholamine uptake inhibitor
- Dopamine uptake system
- Na-dependence
ASJC Scopus subject areas
- Neuroscience(all)