Sodium independence of the binding of [3H]GBR 12783 and other dopamine uptake inhibitors to the dopamine uptake complex

Saloua Benmansour, Jean Jacques Bonnet, Philippe Protais, Jean Costentin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalNeuroscience Letters
Volume77
Issue number1
DOIs
StatePublished - Jun 1 1987
Externally publishedYes

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Dopamine Uptake Inhibitors
Dopamine Plasma Membrane Transport Proteins
Sodium
amineptin
Mazindol
Nomifensine
Ions
Corpus Striatum
Tromethamine
Catecholamines
Cations
Binding Sites
Membranes
1-(2-(diphenylmethoxy)ethyl)-4-(3-phenyl-2-propenyl)piperazine

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sodium independence of the binding of [3H]GBR 12783 and other dopamine uptake inhibitors to the dopamine uptake complex. / Benmansour, Saloua; Bonnet, Jean Jacques; Protais, Philippe; Costentin, Jean.

In: Neuroscience Letters, Vol. 77, No. 1, 01.06.1987, p. 97-102.

Research output: Contribution to journalArticle

Benmansour, Saloua ; Bonnet, Jean Jacques ; Protais, Philippe ; Costentin, Jean. / Sodium independence of the binding of [3H]GBR 12783 and other dopamine uptake inhibitors to the dopamine uptake complex. In: Neuroscience Letters. 1987 ; Vol. 77, No. 1. pp. 97-102.
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abstract = "When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.",
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N2 - When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.

AB - When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.

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