Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 97-102 |
| Number of pages | 6 |
| Journal | Neuroscience Letters |
| Volume | 77 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 1 1987 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Sodium independence of the binding of [3H]GBR 12783 and other dopamine uptake inhibitors to the dopamine uptake complex. / Benmansour, Saloua; Bonnet, Jean Jacques; Protais, Philippe; Costentin, Jean.
In: Neuroscience Letters, Vol. 77, No. 1, 01.06.1987, p. 97-102.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sodium independence of the binding of [3H]GBR 12783 and other dopamine uptake inhibitors to the dopamine uptake complex
AU - Benmansour, Saloua
AU - Bonnet, Jean Jacques
AU - Protais, Philippe
AU - Costentin, Jean
PY - 1987/6/1
Y1 - 1987/6/1
N2 - When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.
AB - When Na+ was the only cation present in the incubation medium used for the determination of the specific binding of [3H]GBR 12783 in rat striatal membranes, the Na+-dependence between 10 and 210 mM Na+ was not observed. In media with low (10 mM) or high (130 mM) Na+ concentration, mazindol and nomifensine competed with [3H]GBR 12783 for its specific binding site with the same affinities. With the exception of amineptine, all the tested catecholamine uptake inhibitors were equally potent to compete with [3H]GBR 12783 when Na+ concentration was decreased from 130 to 10 mM. These data suggest that the media previously used for the binding studies of tritiated inhibitors of dopamine uptake (Tris-ions buffer and Krebs-Ringer medium) contain ions which could exert inhibitory effects on the specific binding at low Na+ concentration.
KW - Binding in vitro
KW - Catecholamine uptake inhibitor
KW - Dopamine uptake system
KW - Na-dependence
UR - http://www.scopus.com/inward/record.url?scp=0023238047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023238047&partnerID=8YFLogxK
U2 - 10.1016/0304-3940(87)90614-8
DO - 10.1016/0304-3940(87)90614-8
M3 - Article
C2 - 3601221
AN - SCOPUS:0023238047
VL - 77
SP - 97
EP - 102
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 1
ER -