Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates

Jaclyn N. Bonner, Koyi Choi, Xiaoyu Xue, Nikko P. Torres, Barnabas Szakal, Lei Wei, Bingbing Wan, Meret Arter, Joao Matos, Patrick Sung, Grant W. Brown, Dana Branzei, Xiaolan Zhao

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Timely removal of DNA recombination intermediates is critical for genome stability. The DNA helicase-topoisomerase complex, Sgs1-Top3-Rmi1 (STR), is the major pathway for processing these intermediates to generate conservative products. However, the mechanisms that promote STR-mediated functions remain to be defined. Here we show that Sgs1 binds to poly-SUMO chains and associates with the Smc5/6 SUMO E3 complex in yeast. Moreover, these interactions contribute to the sumoylation of Sgs1, Top3, and Rmi1 upon the generation of recombination structures. We show that reduced STR sumoylation leads to accumulation of recombination structures, and impaired growth in conditions when these structures arise frequently, highlighting the importance of STR sumoylation. Mechanistically, sumoylation promotes STR inter-subunit interactions and accumulation at DNA repair centers. These findings expand the roles of sumoylation and Smc5/6 in genome maintenance by demonstrating that they foster STR functions in the removal of recombination intermediates.

Original languageEnglish (US)
Pages (from-to)368-378
Number of pages11
JournalCell Reports
Volume16
Issue number2
DOIs
StatePublished - Jul 12 2016
Externally publishedYes

Keywords

  • Sgs1
  • Smc5/6
  • protein sumoylation
  • recombination intermediates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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