Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C

Sara R. Selitsky, Jeanette Baran-Gale, Masao Honda, Daisuke Yamane, Takahiro Masaki, Emily E. Fannin, Bernadette Guerra, Takayoshi Shirasaki, Tetsuro Shimakami, Shuichi Kaneko, Robert E. Lanford, Stanley M. Lemon, Praveen Sethupathy

    Research output: Contribution to journalArticlepeer-review

    73 Scopus citations

    Abstract

    Persistent infections with hepatitis B virus (HBV) or hepatitis C virus (HCV) account for the majority of cases of hepatic cirrhosis and hepatocellular carcinoma (HCC) worldwide. Small, non-coding RNAs play important roles in virus-host interactions. We used high throughput sequencing to conduct an unbiased profiling of small (14-40nts) RNAs in liver from Japanese subjects with advanced hepatitis B or C and hepatocellular carcinoma (HCC). Small RNAs derived from tRNAs, specifically 30-35 nucleotide-long 5′ tRNA-halves (5′ tRHs), were abundant in non-malignant liver and significantly increased in humans and chimpanzees with chronic viral hepatitis. 5′ tRH abundance exceeded microRNA abundance in most infected non-cancerous tissues. In contrast, in matched cancer tissue, 5′ tRH abundance was reduced, and relative abundance of individual 5′ tRHs was altered. In hepatitis B-associated HCC, 5′ tRH abundance correlated with expression of the tRNA-cleaving ribonuclease, angiogenin. These results demonstrate that tRHs are the most abundant small RNAs in chronically infected liver and that their abundance is altered in liver cancer.

    Original languageEnglish (US)
    Article number7675
    JournalScientific reports
    Volume5
    DOIs
    StatePublished - Jan 8 2015

    ASJC Scopus subject areas

    • General

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