Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C

Sara R. Selitsky; Jeanette Baran-Gale; Masao Honda; Daisuke Yamane; Takahiro Masaki; Emily E. Fannin; Bernadette Guerra; Takayoshi Shirasaki; Tetsuro Shimakami; Shuichi Kaneko; Robert E. Lanford; Stanley M. Lemon; Praveen Sethupathy

doi:10.1038/srep07675

Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C

Sara R. Selitsky, Jeanette Baran-Gale, Masao Honda, Daisuke Yamane, Takahiro Masaki, Emily E. Fannin, Bernadette Guerra, Takayoshi Shirasaki, Tetsuro Shimakami, Shuichi Kaneko, Robert E. Lanford, Stanley M. Lemon, Praveen Sethupathy

Research output: Contribution to journal › Article › peer-review

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Abstract

Persistent infections with hepatitis B virus (HBV) or hepatitis C virus (HCV) account for the majority of cases of hepatic cirrhosis and hepatocellular carcinoma (HCC) worldwide. Small, non-coding RNAs play important roles in virus-host interactions. We used high throughput sequencing to conduct an unbiased profiling of small (14-40nts) RNAs in liver from Japanese subjects with advanced hepatitis B or C and hepatocellular carcinoma (HCC). Small RNAs derived from tRNAs, specifically 30-35 nucleotide-long 5′ tRNA-halves (5′ tRHs), were abundant in non-malignant liver and significantly increased in humans and chimpanzees with chronic viral hepatitis. 5′ tRH abundance exceeded microRNA abundance in most infected non-cancerous tissues. In contrast, in matched cancer tissue, 5′ tRH abundance was reduced, and relative abundance of individual 5′ tRHs was altered. In hepatitis B-associated HCC, 5′ tRH abundance correlated with expression of the tRNA-cleaving ribonuclease, angiogenin. These results demonstrate that tRHs are the most abundant small RNAs in chronically infected liver and that their abundance is altered in liver cancer.

Original language	English (US)
Article number	7675
Journal	Scientific reports
Volume	5
DOIs	https://doi.org/10.1038/srep07675
State	Published - Jan 8 2015
Externally published	Yes

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@article{6880e29a78824e4fa6d351a24ac8e6c6,

title = "Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C",

abstract = "Persistent infections with hepatitis B virus (HBV) or hepatitis C virus (HCV) account for the majority of cases of hepatic cirrhosis and hepatocellular carcinoma (HCC) worldwide. Small, non-coding RNAs play important roles in virus-host interactions. We used high throughput sequencing to conduct an unbiased profiling of small (14-40nts) RNAs in liver from Japanese subjects with advanced hepatitis B or C and hepatocellular carcinoma (HCC). Small RNAs derived from tRNAs, specifically 30-35 nucleotide-long 5′ tRNA-halves (5′ tRHs), were abundant in non-malignant liver and significantly increased in humans and chimpanzees with chronic viral hepatitis. 5′ tRH abundance exceeded microRNA abundance in most infected non-cancerous tissues. In contrast, in matched cancer tissue, 5′ tRH abundance was reduced, and relative abundance of individual 5′ tRHs was altered. In hepatitis B-associated HCC, 5′ tRH abundance correlated with expression of the tRNA-cleaving ribonuclease, angiogenin. These results demonstrate that tRHs are the most abundant small RNAs in chronically infected liver and that their abundance is altered in liver cancer.",

author = "Selitsky, {Sara R.} and Jeanette Baran-Gale and Masao Honda and Daisuke Yamane and Takahiro Masaki and Fannin, {Emily E.} and Bernadette Guerra and Takayoshi Shirasaki and Tetsuro Shimakami and Shuichi Kaneko and Lanford, {Robert E.} and Lemon, {Stanley M.} and Praveen Sethupathy",

note = "Funding Information: This work was supported by grants from the National Institutes of Health: R00-DK091318 (P.S.); R01-AI095690 and R01-CA164029 (S.M.L); T32-GM067553 and T32-AI007419 (S.R.S). The Southwest National Primate Research Center is supported by a grant from the NIH Office of Research Infrastructure Programs/OD P51 OD011133), and by Research Facilities Improvement Program Grants C06 RR 12087 and C06 RR016228.",

year = "2015",

month = jan,

day = "8",

doi = "10.1038/srep07675",

language = "English (US)",

volume = "5",

journal = "Scientific Reports",

issn = "2045-2322",

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T1 - Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C

AU - Selitsky, Sara R.

AU - Baran-Gale, Jeanette

AU - Honda, Masao

AU - Yamane, Daisuke

AU - Masaki, Takahiro

AU - Fannin, Emily E.

AU - Guerra, Bernadette

AU - Shirasaki, Takayoshi

AU - Shimakami, Tetsuro

AU - Kaneko, Shuichi

AU - Lanford, Robert E.

AU - Lemon, Stanley M.

AU - Sethupathy, Praveen

N1 - Funding Information: This work was supported by grants from the National Institutes of Health: R00-DK091318 (P.S.); R01-AI095690 and R01-CA164029 (S.M.L); T32-GM067553 and T32-AI007419 (S.R.S). The Southwest National Primate Research Center is supported by a grant from the NIH Office of Research Infrastructure Programs/OD P51 OD011133), and by Research Facilities Improvement Program Grants C06 RR 12087 and C06 RR016228.

PY - 2015/1/8

Y1 - 2015/1/8

N2 - Persistent infections with hepatitis B virus (HBV) or hepatitis C virus (HCV) account for the majority of cases of hepatic cirrhosis and hepatocellular carcinoma (HCC) worldwide. Small, non-coding RNAs play important roles in virus-host interactions. We used high throughput sequencing to conduct an unbiased profiling of small (14-40nts) RNAs in liver from Japanese subjects with advanced hepatitis B or C and hepatocellular carcinoma (HCC). Small RNAs derived from tRNAs, specifically 30-35 nucleotide-long 5′ tRNA-halves (5′ tRHs), were abundant in non-malignant liver and significantly increased in humans and chimpanzees with chronic viral hepatitis. 5′ tRH abundance exceeded microRNA abundance in most infected non-cancerous tissues. In contrast, in matched cancer tissue, 5′ tRH abundance was reduced, and relative abundance of individual 5′ tRHs was altered. In hepatitis B-associated HCC, 5′ tRH abundance correlated with expression of the tRNA-cleaving ribonuclease, angiogenin. These results demonstrate that tRHs are the most abundant small RNAs in chronically infected liver and that their abundance is altered in liver cancer.

AB - Persistent infections with hepatitis B virus (HBV) or hepatitis C virus (HCV) account for the majority of cases of hepatic cirrhosis and hepatocellular carcinoma (HCC) worldwide. Small, non-coding RNAs play important roles in virus-host interactions. We used high throughput sequencing to conduct an unbiased profiling of small (14-40nts) RNAs in liver from Japanese subjects with advanced hepatitis B or C and hepatocellular carcinoma (HCC). Small RNAs derived from tRNAs, specifically 30-35 nucleotide-long 5′ tRNA-halves (5′ tRHs), were abundant in non-malignant liver and significantly increased in humans and chimpanzees with chronic viral hepatitis. 5′ tRH abundance exceeded microRNA abundance in most infected non-cancerous tissues. In contrast, in matched cancer tissue, 5′ tRH abundance was reduced, and relative abundance of individual 5′ tRHs was altered. In hepatitis B-associated HCC, 5′ tRH abundance correlated with expression of the tRNA-cleaving ribonuclease, angiogenin. These results demonstrate that tRHs are the most abundant small RNAs in chronically infected liver and that their abundance is altered in liver cancer.

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ER -

Small tRNA-derived RNAs are increased and more abundant than microRNAs in chronic hepatitis B and C

Abstract

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