Small-molecule high-throughput screening utilizing xenopus egg extract

Matthew R. Broadus, Patricia R Yew, Stephen R. Hann, Ethan Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Screens for small-molecule modulators of biological pathways typically utilize cultured cell lines, purified proteins, or, recently, model organisms (e.g., zebrafish, Drosophila, C. elegans ). Herein, we describe a method for using Xenopus laevis egg extract, a biologically active and highly tractable cell-free system that recapitulates a legion of complex chemical reactions found in intact cells. Specifically, we focus on the use of a luciferase-based fusion system to identify small-molecule modulators that affect protein turnover.

Original languageEnglish (US)
Pages (from-to)63-73
Number of pages11
JournalMethods in Molecular Biology
Volume1263
DOIs
StatePublished - 2015
Externally publishedYes

Fingerprint

Xenopus
Ovum
Cell-Free System
Xenopus laevis
Zebrafish
Luciferases
Drosophila
Cultured Cells
Proteins
Cell Line

Keywords

  • Cell-free
  • High-throughput screening
  • Protein degradation
  • Protein turnover
  • Small molecules
  • Xenopus egg extract
  • Xenopus laevis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Small-molecule high-throughput screening utilizing xenopus egg extract. / Broadus, Matthew R.; Yew, Patricia R; Hann, Stephen R.; Lee, Ethan.

In: Methods in Molecular Biology, Vol. 1263, 2015, p. 63-73.

Research output: Contribution to journalArticle

Broadus, Matthew R. ; Yew, Patricia R ; Hann, Stephen R. ; Lee, Ethan. / Small-molecule high-throughput screening utilizing xenopus egg extract. In: Methods in Molecular Biology. 2015 ; Vol. 1263. pp. 63-73.
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