Slowed recovery of rod photoresponse in mice lacking the GTPase accelerating protein RGS9-1

Ching Kang Chen, Marie E. Burns, Wel He, Theodorø G. Wensel, Denis A. Baylor, Melvin I. Simon

Research output: Contribution to journalArticlepeer-review

338 Scopus citations


Timely deactivation of the α-subunit of the rod G-protein trans-ducin (Gαt) is essential for the temporal resolution of rod vision. Regulators of G-protein signalling (RGS) proteins accelerate hydrolysis of GTP by the α- subunits of heterotrimeric G proteins in vitro. Several retinal RGS proteins can act in vitro as GTPase accelerating proteins (GAP) for Gαt. Recent reconstitution experiments indicate that one of these, RGS9-1, may account for much of the Gαt GAP activity in rod outer segments (ROS). Here we report that ROS membranes from mice lacking RGS9-1 hydrolyse GTP more slowly than ROS membranes from control mice. The Gβ5-L protein that forms a complex with RGS9-1 (ref. 10) was absent from RGS9(-/-) retinas, although Gβ5-L messenger RNA was still present. The flash responses of RGS9(-/-) rods rose normally, but recovered much more slowly than normal. We conclude that RGS9-1, probably in a complex with Gβ5-L, is essential for acceleration of hydrolysis of GTP by Gαt and for normal recovery of the photoresponse.

Original languageEnglish (US)
Pages (from-to)557-560
Number of pages4
Issue number6769
StatePublished - Feb 3 2000
Externally publishedYes

ASJC Scopus subject areas

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