TY - JOUR
T1 - Skeletal muscle satellite cell activation following cutaneous burn in rats
AU - Wu, Xiaowu
AU - Walters, Thomas J.
AU - Rathbone, Christopher R.
N1 - Funding Information:
We thank Ms. Melissa Sanchez for expert technical assistance. Research was supported by United States Army Medical Research and Materiel Command. The opinions or assertions contained herein are the private views of the author and are not to be construed as official or reflecting the views of the US Department of Defense or the US Government. The author is an employee of the US Government. This work was prepared as part of his official duties and, as such, there is no copyright to be transferred.
PY - 2013
Y1 - 2013
N2 - Background: Cutaneous burn distant from skeletal muscles induces atrophy; however, its effect on muscle stem cells resident in skeletal muscle (satellite cells) distal to burn is not known. Methods: Satellite cell activation was measured in predominantly fast-twitch [tibialis anterior, extensor digitorum longus (EDL), plantaris, and gastrocnemius] and slow-twitch (soleus) muscles of rats that received either 40% total body surface area full-thickness scald burn or sham burn to the trunk area by determining bromodeoxyuridine incorporation, MyoD, and Pax7 immunohistochemistry in vivo ≤48 h after burn. To determine the effects of circulating factors on satellite cell activation, satellite cell cultures were treated with serum from sham or burn rats. Results: In vivo activation of satellite cells was increased in fast muscles isolated from burn as compared to sham animals, whereas a significant response was not seen in slow muscles. Serum taken from animals in the burn group increased the activation of satellite cells isolated from both sham and burn animals in vitro, suggesting that circulating factors have the potential to increase satellite cell activation following burn. Conclusions: Increases in satellite cell activation in muscles distal to burn are fiber-type-dependent, and circulating factors may play a role in the activation of satellite cells following burn. A better understanding of the impact of burn on satellite cell functionality will allow us to identify the cellular mechanisms of long-term muscle atrophy.
AB - Background: Cutaneous burn distant from skeletal muscles induces atrophy; however, its effect on muscle stem cells resident in skeletal muscle (satellite cells) distal to burn is not known. Methods: Satellite cell activation was measured in predominantly fast-twitch [tibialis anterior, extensor digitorum longus (EDL), plantaris, and gastrocnemius] and slow-twitch (soleus) muscles of rats that received either 40% total body surface area full-thickness scald burn or sham burn to the trunk area by determining bromodeoxyuridine incorporation, MyoD, and Pax7 immunohistochemistry in vivo ≤48 h after burn. To determine the effects of circulating factors on satellite cell activation, satellite cell cultures were treated with serum from sham or burn rats. Results: In vivo activation of satellite cells was increased in fast muscles isolated from burn as compared to sham animals, whereas a significant response was not seen in slow muscles. Serum taken from animals in the burn group increased the activation of satellite cells isolated from both sham and burn animals in vitro, suggesting that circulating factors have the potential to increase satellite cell activation following burn. Conclusions: Increases in satellite cell activation in muscles distal to burn are fiber-type-dependent, and circulating factors may play a role in the activation of satellite cells following burn. A better understanding of the impact of burn on satellite cell functionality will allow us to identify the cellular mechanisms of long-term muscle atrophy.
KW - Activation
KW - Atrophy
KW - Muscle precursor cell
KW - Skeletal muscle
KW - Thermal injury
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U2 - 10.1016/j.burns.2012.10.016
DO - 10.1016/j.burns.2012.10.016
M3 - Article
C2 - 23146573
AN - SCOPUS:84877088118
VL - 39
SP - 736
EP - 744
JO - Burns
JF - Burns
SN - 0305-4179
IS - 4
ER -