Sites of mercury inhibition of amino acid transport in fish gut

A. Farmanfarmaian, Luzhe Sun, K. Pugliese, P. Hannoush

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In polluted waters, mercury accumulation in the intestine and other tissues of marine and estuarine fish can reach several orders of magnitude above those found in the water column. Entrance of mercury compounds, especially HgCl2, via food and drink into the lumen of the gut can inhibit the normal absorption of amino acids and sugars.1-5 When applied as HgCl2 to the mucosal side of fish intestine, mercury binds strongly to the apical membrane facing the lumen and is transferred at a low rate across the gut epithelium to the blood.6-8 The site(s) of mercury inhibition of amino acid absorption may be on the luminal face of the apical membrane, intracellular, or on the basolateral membrane facing the interstitial fluid in equilibrium with blood. A combination of these sites is not ruled out. The relative effect of the application of HgCl2 to the mucosal, and separately, to the serosal sides of the gut during leucine transport was investigated. 14C-Leucine transfer in toadfish midgut was measured in two sets of Ussing chambers.9 The results showed a 70-74% inhibition of net mucosal-to-serosal (M → S) transfer and an 89% inhibition of tissue accumulation when HgCl2 was on the mucosal side. In the parallel series, when HgCl2 was applied on the serosal side only, the net mucosal-to-serosal transfer was reduced by 48-52% while tissue accumulation was only slightly below the control. Tissues preloaded with 14C-leucine were investigated with respect to cell-to-mucosal (→ M) or cell-to-serosal (C → S) efflux. Mucosal HgCl2 had a minimal and complex inhibitory effect on C → M efflux and no effect on C → S efflux. By contrast, serosal HgCl2 had no effect on C → M efflux and caused only a weak reduction in C → S efflux. These results indicate that HgCl2 inhibits amino acid transport mainly by blocking transfer channels at the extracellular face of the apical (mucosal), and to a lesser degree, basolateral (serosal) membrane of the gut epithelium.

Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalMarine Environmental Research
Volume24
Issue number1-4
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

mercury
Fish
Amino acids
digestive system
amino acid
Tissue
membrane
leucine
Membranes
amino acids
fish
intestines
mercury compounds
epithelium
Mercury compounds
cells
Batrachoididae
estuarine fish
extracellular fluids
midgut

ASJC Scopus subject areas

  • Aquatic Science
  • Oceanography
  • Environmental Science(all)

Cite this

Sites of mercury inhibition of amino acid transport in fish gut. / Farmanfarmaian, A.; Sun, Luzhe; Pugliese, K.; Hannoush, P.

In: Marine Environmental Research, Vol. 24, No. 1-4, 1988, p. 203-206.

Research output: Contribution to journalArticle

Farmanfarmaian, A. ; Sun, Luzhe ; Pugliese, K. ; Hannoush, P. / Sites of mercury inhibition of amino acid transport in fish gut. In: Marine Environmental Research. 1988 ; Vol. 24, No. 1-4. pp. 203-206.
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N2 - In polluted waters, mercury accumulation in the intestine and other tissues of marine and estuarine fish can reach several orders of magnitude above those found in the water column. Entrance of mercury compounds, especially HgCl2, via food and drink into the lumen of the gut can inhibit the normal absorption of amino acids and sugars.1-5 When applied as HgCl2 to the mucosal side of fish intestine, mercury binds strongly to the apical membrane facing the lumen and is transferred at a low rate across the gut epithelium to the blood.6-8 The site(s) of mercury inhibition of amino acid absorption may be on the luminal face of the apical membrane, intracellular, or on the basolateral membrane facing the interstitial fluid in equilibrium with blood. A combination of these sites is not ruled out. The relative effect of the application of HgCl2 to the mucosal, and separately, to the serosal sides of the gut during leucine transport was investigated. 14C-Leucine transfer in toadfish midgut was measured in two sets of Ussing chambers.9 The results showed a 70-74% inhibition of net mucosal-to-serosal (M → S) transfer and an 89% inhibition of tissue accumulation when HgCl2 was on the mucosal side. In the parallel series, when HgCl2 was applied on the serosal side only, the net mucosal-to-serosal transfer was reduced by 48-52% while tissue accumulation was only slightly below the control. Tissues preloaded with 14C-leucine were investigated with respect to cell-to-mucosal (→ M) or cell-to-serosal (C → S) efflux. Mucosal HgCl2 had a minimal and complex inhibitory effect on C → M efflux and no effect on C → S efflux. By contrast, serosal HgCl2 had no effect on C → M efflux and caused only a weak reduction in C → S efflux. These results indicate that HgCl2 inhibits amino acid transport mainly by blocking transfer channels at the extracellular face of the apical (mucosal), and to a lesser degree, basolateral (serosal) membrane of the gut epithelium.

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