Site-Directed Delivery of VEGF-Targeted Liposomes into Intracranial C6 Glioma

S. A. Shein; N. V. Nukolova; A. A. Korchagina; T. O. Abakumova; I. I. Kiuznetsov; M. A. Abakumov; V. P. Baklaushev; O. I. Gurina; V. P. Chekhonin

doi:10.1007/s10517-015-2765-4

Site-Directed Delivery of VEGF-Targeted Liposomes into Intracranial C6 Glioma

S. A. Shein, N. V. Nukolova, A. A. Korchagina, T. O. Abakumova, I. I. Kiuznetsov, M. A. Abakumov, V. P. Baklaushev, O. I. Gurina, V. P. Chekhonin

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The efficiency of conventional chemotherapy for aggressive tumors in the CNS remains low and new strategies for the targeted delivery of anti-tumor substances are now actively developed. Pegylated liposomes covalently conjugated with monoclonal antibodies to VEGF synthesized by us are nanoparticle characterized by narrow size distribution and high dispersion stability. Immunochemical activity of antibodies after conjugation was 70% of initial level. The anti-VEGF liposomes developed by us were highly specific for VEGF⁺ tumor cells (in vitro and in vivo). Intravenous injection of VEGF-liposomes to rats with intracranial C6 glioma was followed by their specific accumulation in the malignant tissues and engulfment by glioma cells, which attested to target delivery and selective accumulation of anti-VEGF-liposomes in the brain tumor. Thus, the use of targeting molecules can significantly increase the distribution and efficiency of delivery of nanocontainers to a tumor characterized by hyperexpression of the target proteins.

Original language	English (US)
Pages (from-to)	371-376
Number of pages	6
Journal	Bulletin of Experimental Biology and Medicine
Volume	158
Issue number	3
DOIs	https://doi.org/10.1007/s10517-015-2765-4
State	Published - Jan 1 2015
Externally published	Yes

Keywords

brain tumors
liposomes
monoclonal antibodies
target delivery
vascular endothelial growth factor (VEGF)

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1007/s10517-015-2765-4

Cite this

@article{525c56726d48447e88f48bd0db62521d,

title = "Site-Directed Delivery of VEGF-Targeted Liposomes into Intracranial C6 Glioma",

abstract = "The efficiency of conventional chemotherapy for aggressive tumors in the CNS remains low and new strategies for the targeted delivery of anti-tumor substances are now actively developed. Pegylated liposomes covalently conjugated with monoclonal antibodies to VEGF synthesized by us are nanoparticle characterized by narrow size distribution and high dispersion stability. Immunochemical activity of antibodies after conjugation was 70% of initial level. The anti-VEGF liposomes developed by us were highly specific for VEGF+ tumor cells (in vitro and in vivo). Intravenous injection of VEGF-liposomes to rats with intracranial C6 glioma was followed by their specific accumulation in the malignant tissues and engulfment by glioma cells, which attested to target delivery and selective accumulation of anti-VEGF-liposomes in the brain tumor. Thus, the use of targeting molecules can significantly increase the distribution and efficiency of delivery of nanocontainers to a tumor characterized by hyperexpression of the target proteins.",

keywords = "brain tumors, liposomes, monoclonal antibodies, target delivery, vascular endothelial growth factor (VEGF)",

author = "Shein, {S. A.} and Nukolova, {N. V.} and Korchagina, {A. A.} and Abakumova, {T. O.} and Kiuznetsov, {I. I.} and Abakumov, {M. A.} and Baklaushev, {V. P.} and Gurina, {O. I.} and Chekhonin, {V. P.}",

note = "Publisher Copyright: {\textcopyright} 2015, Springer Science+Business Media New York.",

year = "2015",

month = jan,

day = "1",

doi = "10.1007/s10517-015-2765-4",

language = "English (US)",

volume = "158",

pages = "371--376",

journal = "Bulletin of Experimental Biology and Medicine",

issn = "0007-4888",

publisher = "Springer",

number = "3",

}

TY - JOUR

T1 - Site-Directed Delivery of VEGF-Targeted Liposomes into Intracranial C6 Glioma

AU - Shein, S. A.

AU - Nukolova, N. V.

AU - Korchagina, A. A.

AU - Abakumova, T. O.

AU - Kiuznetsov, I. I.

AU - Abakumov, M. A.

AU - Baklaushev, V. P.

AU - Gurina, O. I.

AU - Chekhonin, V. P.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - The efficiency of conventional chemotherapy for aggressive tumors in the CNS remains low and new strategies for the targeted delivery of anti-tumor substances are now actively developed. Pegylated liposomes covalently conjugated with monoclonal antibodies to VEGF synthesized by us are nanoparticle characterized by narrow size distribution and high dispersion stability. Immunochemical activity of antibodies after conjugation was 70% of initial level. The anti-VEGF liposomes developed by us were highly specific for VEGF+ tumor cells (in vitro and in vivo). Intravenous injection of VEGF-liposomes to rats with intracranial C6 glioma was followed by their specific accumulation in the malignant tissues and engulfment by glioma cells, which attested to target delivery and selective accumulation of anti-VEGF-liposomes in the brain tumor. Thus, the use of targeting molecules can significantly increase the distribution and efficiency of delivery of nanocontainers to a tumor characterized by hyperexpression of the target proteins.

AB - The efficiency of conventional chemotherapy for aggressive tumors in the CNS remains low and new strategies for the targeted delivery of anti-tumor substances are now actively developed. Pegylated liposomes covalently conjugated with monoclonal antibodies to VEGF synthesized by us are nanoparticle characterized by narrow size distribution and high dispersion stability. Immunochemical activity of antibodies after conjugation was 70% of initial level. The anti-VEGF liposomes developed by us were highly specific for VEGF+ tumor cells (in vitro and in vivo). Intravenous injection of VEGF-liposomes to rats with intracranial C6 glioma was followed by their specific accumulation in the malignant tissues and engulfment by glioma cells, which attested to target delivery and selective accumulation of anti-VEGF-liposomes in the brain tumor. Thus, the use of targeting molecules can significantly increase the distribution and efficiency of delivery of nanocontainers to a tumor characterized by hyperexpression of the target proteins.

KW - brain tumors

KW - liposomes

KW - monoclonal antibodies

KW - target delivery

KW - vascular endothelial growth factor (VEGF)

UR - http://www.scopus.com/inward/record.url?scp=84939958326&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939958326&partnerID=8YFLogxK

U2 - 10.1007/s10517-015-2765-4

DO - 10.1007/s10517-015-2765-4

M3 - Article

C2 - 25573371

AN - SCOPUS:84939958326

SN - 0007-4888

VL - 158

SP - 371

EP - 376

JO - Bulletin of Experimental Biology and Medicine

JF - Bulletin of Experimental Biology and Medicine

IS - 3

ER -

Site-Directed Delivery of VEGF-Targeted Liposomes into Intracranial C6 Glioma

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this