Single-step method to prepare native angiostatin from human plasma

Fu Yang Li, Peng He, Xin Ping Liu, Ying Qi Zhang, Jing Hua Yang, Dai Ming Fan, Li Bo Yao

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

As a specific angiogenesis inhibitor, angiostatin can inhibit new vascular formation, and it has potential clinical practical value. Human plasminogen was purified from human plasma by affinity chromatograph, and then in situ digestion of plasminogen by elastase was carried out to produce angiostatin fragments. After washing, angiostain was eluted specifically by 0.2 mol/L 6-aminocaproic acid solution. This improvement made this method be simpler, rapider and more efficient. In vitro and in vivo experiments indicated that this purified protein has potent antiangiogenic activity.

Original languageEnglish (US)
Pages (from-to)327
Number of pages1
JournalProgress in Biochemistry and Biophysics
Volume27
Issue number3
StatePublished - 2000
Externally publishedYes

Fingerprint

Angiostatins
Plasma (human)
Plasminogen
Aminocaproic Acid
Angiogenesis Inhibitors
Pancreatic Elastase
Washing
Blood Vessels
Digestion
Proteins
Experiments

Keywords

  • Angiogenesis
  • Angiostatin
  • Protein
  • Purification

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Li, F. Y., He, P., Liu, X. P., Zhang, Y. Q., Yang, J. H., Fan, D. M., & Yao, L. B. (2000). Single-step method to prepare native angiostatin from human plasma. Progress in Biochemistry and Biophysics, 27(3), 327.

Single-step method to prepare native angiostatin from human plasma. / Li, Fu Yang; He, Peng; Liu, Xin Ping; Zhang, Ying Qi; Yang, Jing Hua; Fan, Dai Ming; Yao, Li Bo.

In: Progress in Biochemistry and Biophysics, Vol. 27, No. 3, 2000, p. 327.

Research output: Contribution to journalArticle

Li, FY, He, P, Liu, XP, Zhang, YQ, Yang, JH, Fan, DM & Yao, LB 2000, 'Single-step method to prepare native angiostatin from human plasma', Progress in Biochemistry and Biophysics, vol. 27, no. 3, pp. 327.
Li FY, He P, Liu XP, Zhang YQ, Yang JH, Fan DM et al. Single-step method to prepare native angiostatin from human plasma. Progress in Biochemistry and Biophysics. 2000;27(3):327.
Li, Fu Yang ; He, Peng ; Liu, Xin Ping ; Zhang, Ying Qi ; Yang, Jing Hua ; Fan, Dai Ming ; Yao, Li Bo. / Single-step method to prepare native angiostatin from human plasma. In: Progress in Biochemistry and Biophysics. 2000 ; Vol. 27, No. 3. pp. 327.
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