Single-site cleavage in the 5′-untranslated region of Leishmaniavirus RNA is mediated by the viral capsid protein

Kyle J. Macbeth, Jean L. Patterson

    Research output: Contribution to journalArticle

    19 Scopus citations

    Abstract

    Leishmaniavirus (LRV) is a double-stranded RNA virus that persistently infects the protozoan parasite Leishmania. LRV produces a short RNA transcript, corresponding to the 5′ end of positive-sense viral RNA, both in vivo and in in vitro polymerase assays. The short transcript is generated by a single site-specific cleavage event in the 5′ untranslated region of the 5.3-kb genome. This cleavage event can be reproduced in vitro with purified viral particles and a substrate RNA transcript possessing the viral cleavage site. A region of nucleotides required for cleavage was identified by analyzing the cleavage sites yielding the short transcripts of various LRV isolates. A 6-nt deletion at this cleavage site completely abolished RNA processing. In an in vitro cleavage assay, baculovirus-expressed capsid protein possessed an endonuclease activity identical to that of native virions, showing that the viral capsid protein is the RNA endonuclease. Identification of the LRV capsid protein as an RNA endonuclease is unprecedented among known viral capsid proteins.

    Original languageEnglish (US)
    Pages (from-to)8994-8998
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume92
    Issue number19
    DOIs
    StatePublished - Sep 12 1995

    Keywords

    • Double-stranded RNA virus
    • Endoribonuclease
    • Leishmania
    • RNA processing
    • Totiviridae

    ASJC Scopus subject areas

    • General

    Fingerprint Dive into the research topics of 'Single-site cleavage in the 5′-untranslated region of Leishmaniavirus RNA is mediated by the viral capsid protein'. Together they form a unique fingerprint.

  • Cite this