Single-particle visualization of assembly: I. Dimerization in a planar zone

H. Wang, I. Wu, Q. Yang, C. E. Catalano, Phillip Serwer

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Single-particle fluorescence microscopy of association/dissociation is required for analysis of biological assembly reactions. Toward achieving this goal, Wang et al. (J. Microsc., 2004, 213, 101-109) used molten agarose to concentrate thermally diffusing particles in a thin zone of solution next to the surface of a coverglass (plane of concentration). The present study details the first real-time, single-particle analysis of the association/dissociation of thermally diffusing particles in the plane of concentration. The test particles were procapsids of bacteriophage λ (radius = 31 nm). Quantification of thermal motion was developed and used to determine whether co-diffusing particles were bound to each other. The data are explained by (1) the presence of a molten agarose-generated barrier that is 93-155 nm from the coverglass surface, and (2) nonrandom orientation of procapsid dimers in the plane of concentration.

Original languageEnglish (US)
Pages (from-to)83-92
Number of pages10
JournalJournal of Microscopy
Volume217
Issue number1
DOIs
StatePublished - Jan 2005

Keywords

  • Alexa
  • Association/dissociation
  • Bacteriophage
  • Capsid
  • Confined thermal motion
  • Dye
  • Fluorescence microscopy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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