TY - JOUR
T1 - Single-particle visualization of assembly
T2 - I. Dimerization in a planar zone
AU - Wang, H.
AU - Wu, I.
AU - Yang, Q.
AU - Catalano, C. E.
AU - Serwer, Phillip
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/1
Y1 - 2005/1
N2 - Single-particle fluorescence microscopy of association/dissociation is required for analysis of biological assembly reactions. Toward achieving this goal, Wang et al. (J. Microsc., 2004, 213, 101-109) used molten agarose to concentrate thermally diffusing particles in a thin zone of solution next to the surface of a coverglass (plane of concentration). The present study details the first real-time, single-particle analysis of the association/dissociation of thermally diffusing particles in the plane of concentration. The test particles were procapsids of bacteriophage λ (radius = 31 nm). Quantification of thermal motion was developed and used to determine whether co-diffusing particles were bound to each other. The data are explained by (1) the presence of a molten agarose-generated barrier that is 93-155 nm from the coverglass surface, and (2) nonrandom orientation of procapsid dimers in the plane of concentration.
AB - Single-particle fluorescence microscopy of association/dissociation is required for analysis of biological assembly reactions. Toward achieving this goal, Wang et al. (J. Microsc., 2004, 213, 101-109) used molten agarose to concentrate thermally diffusing particles in a thin zone of solution next to the surface of a coverglass (plane of concentration). The present study details the first real-time, single-particle analysis of the association/dissociation of thermally diffusing particles in the plane of concentration. The test particles were procapsids of bacteriophage λ (radius = 31 nm). Quantification of thermal motion was developed and used to determine whether co-diffusing particles were bound to each other. The data are explained by (1) the presence of a molten agarose-generated barrier that is 93-155 nm from the coverglass surface, and (2) nonrandom orientation of procapsid dimers in the plane of concentration.
KW - Alexa
KW - Association/dissociation
KW - Bacteriophage
KW - Capsid
KW - Confined thermal motion
KW - Dye
KW - Fluorescence microscopy
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U2 - 10.1111/j.0022-2720.2005.01438.x
DO - 10.1111/j.0022-2720.2005.01438.x
M3 - Article
C2 - 15655066
AN - SCOPUS:13244283065
VL - 217
SP - 83
EP - 92
JO - The Microscopic Journal and Structural Record
JF - The Microscopic Journal and Structural Record
SN - 0022-2720
IS - 1
ER -