Single crystals of bacteriophage T7 RNA polymerase

Rui Sousa; John P. Rose; Yong Je Chung; Eileen M. Lafer; Bi‐Cheng ‐C Wang

doi:10.1002/prot.340050403

Single crystals of bacteriophage T7 RNA polymerase

Rui Sousa, John P. Rose, Yong Je Chung, Eileen M. Lafer, Bi‐Cheng ‐C Wang

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2₁ and have unit cell parameters a =114.5 Å, b=139.6 Å, c=125.7 Å, β=98.1° Self‐rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 Å resolution. These are the first crystals of a DNA‐dependent RNA polymerase suitable for high‐resolution X‐ray structure determination.

Original language	English (US)
Pages (from-to)	266-270
Number of pages	5
Journal	Proteins: Structure, Function, and Bioinformatics
Volume	5
Issue number	4
DOIs	https://doi.org/10.1002/prot.340050403
State	Published - 1989

Keywords

X‐ray diffraction
crystallization
crystals
purification

ASJC Scopus subject areas

Structural Biology
Biochemistry
Molecular Biology

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10.1002/prot.340050403

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title = "Single crystals of bacteriophage T7 RNA polymerase",

abstract = "Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P21 and have unit cell parameters a =114.5 {\AA}, b=139.6 {\AA}, c=125.7 {\AA}, β=98.1° Self‐rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 {\AA} resolution. These are the first crystals of a DNA‐dependent RNA polymerase suitable for high‐resolution X‐ray structure determination.",

keywords = "X‐ray diffraction, crystallization, crystals, purification",

author = "Rui Sousa and Rose, {John P.} and {Je Chung}, Yong and Lafer, {Eileen M.} and Wang, {Bi‐Cheng ‐C}",

year = "1989",

doi = "10.1002/prot.340050403",

language = "English (US)",

volume = "5",

pages = "266--270",

journal = "Proteins: Structure, Function and Bioinformatics",

issn = "0887-3585",

publisher = "Wiley-Liss Inc.",

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TY - JOUR

T1 - Single crystals of bacteriophage T7 RNA polymerase

AU - Sousa, Rui

AU - Rose, John P.

AU - Je Chung, Yong

AU - Lafer, Eileen M.

AU - Wang, Bi‐Cheng ‐C

PY - 1989

Y1 - 1989

N2 - Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P21 and have unit cell parameters a =114.5 Å, b=139.6 Å, c=125.7 Å, β=98.1° Self‐rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 Å resolution. These are the first crystals of a DNA‐dependent RNA polymerase suitable for high‐resolution X‐ray structure determination.

AB - Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P21 and have unit cell parameters a =114.5 Å, b=139.6 Å, c=125.7 Å, β=98.1° Self‐rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 Å resolution. These are the first crystals of a DNA‐dependent RNA polymerase suitable for high‐resolution X‐ray structure determination.

KW - X‐ray diffraction

KW - crystallization

KW - crystals

KW - purification

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DO - 10.1002/prot.340050403

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JO - Proteins: Structure, Function and Bioinformatics

JF - Proteins: Structure, Function and Bioinformatics

SN - 0887-3585

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ER -

Single crystals of bacteriophage T7 RNA polymerase

Abstract

Keywords

ASJC Scopus subject areas

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