Single crystals of bacteriophage T7 RNA polymerase.

Rui J Sousa, J. P. Rose, Y. J. Chung, Eileen M Lafer, B. C. Wang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.

Original languageEnglish (US)
Pages (from-to)266-270
Number of pages5
JournalProteins
Volume5
Issue number4
StatePublished - 1989
Externally publishedYes

Fingerprint

Single crystals
Crystals
DNA-Directed RNA Polymerases
X-Rays
Dissolution
X rays
Molecules
bacteriophage T7 RNA polymerase

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology

Cite this

Sousa, R. J., Rose, J. P., Chung, Y. J., Lafer, E. M., & Wang, B. C. (1989). Single crystals of bacteriophage T7 RNA polymerase. Proteins, 5(4), 266-270.

Single crystals of bacteriophage T7 RNA polymerase. / Sousa, Rui J; Rose, J. P.; Chung, Y. J.; Lafer, Eileen M; Wang, B. C.

In: Proteins, Vol. 5, No. 4, 1989, p. 266-270.

Research output: Contribution to journalArticle

Sousa, RJ, Rose, JP, Chung, YJ, Lafer, EM & Wang, BC 1989, 'Single crystals of bacteriophage T7 RNA polymerase.', Proteins, vol. 5, no. 4, pp. 266-270.
Sousa, Rui J ; Rose, J. P. ; Chung, Y. J. ; Lafer, Eileen M ; Wang, B. C. / Single crystals of bacteriophage T7 RNA polymerase. In: Proteins. 1989 ; Vol. 5, No. 4. pp. 266-270.
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