Single crystals of bacteriophage T7 RNA polymerase

Rui Sousa, John P. Rose, Yong Je Chung, Eileen M. Lafer, Bi‐Cheng ‐C Wang

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P21 and have unit cell parameters a =114.5 Å, b=139.6 Å, c=125.7 Å, β=98.1° Self‐rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 Å resolution. These are the first crystals of a DNA‐dependent RNA polymerase suitable for high‐resolution X‐ray structure determination.

Original languageEnglish (US)
Pages (from-to)266-270
Number of pages5
JournalProteins: Structure, Function, and Bioinformatics
Volume5
Issue number4
DOIs
StatePublished - 1989

Keywords

  • X‐ray diffraction
  • crystallization
  • crystals
  • purification

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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