Abstract
Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 266-270 |
| Number of pages | 5 |
| Journal | Proteins |
| Volume | 5 |
| Issue number | 4 |
| State | Published - 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Genetics
- Structural Biology
Cite this
Single crystals of bacteriophage T7 RNA polymerase. / Sousa, Rui J; Rose, J. P.; Chung, Y. J.; Lafer, Eileen M; Wang, B. C.
In: Proteins, Vol. 5, No. 4, 1989, p. 266-270.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single crystals of bacteriophage T7 RNA polymerase.
AU - Sousa, Rui J
AU - Rose, J. P.
AU - Chung, Y. J.
AU - Lafer, Eileen M
AU - Wang, B. C.
PY - 1989
Y1 - 1989
N2 - Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.
AB - Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 x 0.3 x 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which is enzymatically active upon dissolution. These crystals belong to the monoclinic space group P2(1) and have unit cell parameters a = 114.5 A, b = 139.6 A, c = 125.7 A, and beta = 98.1 degrees. Self-rotation function studies indicate that there are three molecules per asymmetric unit. The crystals diffract to at least 3.0 A resolution. These are the first crystals of a DNA-dependent RNA polymerase suitable for high-resolution X-ray structure determination.
UR - http://www.scopus.com/inward/record.url?scp=0024407827&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024407827&partnerID=8YFLogxK
M3 - Article
C2 - 2678097
AN - SCOPUS:0024407827
VL - 5
SP - 266
EP - 270
JO - Proteins: Structure, Function and Bioinformatics
JF - Proteins: Structure, Function and Bioinformatics
SN - 0887-3585
IS - 4
ER -