Abstract
Single crystals of T7 RNA polymerase have been grown to a maximum size of 1.8 × 0.3 × 0.3 mm. The crystals are composed of fully intact T7 RNA polymerase which in enzymatically active upon dissolution. These crystals belong to the monoclinic space group P21 and have unit cell parameters a =114.5 Å, b=139.6 Å, c=125.7 Å, β=98.1° Self‐rotation function studies indicate that there are three molecules per asymmetricunit. The crystals diffract to at least 3.0 Å resolution. These are the first crystals of a DNA‐dependent RNA polymerase suitable for high‐resolution X‐ray structure determination.
Original language | English (US) |
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Pages (from-to) | 266-270 |
Number of pages | 5 |
Journal | Proteins: Structure, Function, and Bioinformatics |
Volume | 5 |
Issue number | 4 |
DOIs | |
State | Published - 1989 |
Externally published | Yes |
Keywords
- X‐ray diffraction
- crystallization
- crystals
- purification
ASJC Scopus subject areas
- Structural Biology
- Biochemistry
- Molecular Biology