Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci

M. J. Zervos, J. E. Patterson, S. Edberg, C. Pierson, C. A. Kauffman, T. S. Mikesell, D. R. Schaberg

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Growth in a single broth microdilution well containing gentamicin at a concentration of 500 μg/ml predicted high-level resistance to gentamicin (MIC, ≥ 2,000 μg/ml) in 505 of 508 clinical isolates of enterococci. Failure to achieve synergistic killing with the combination of penicillin and an aminoglycoside was demonstrated with 100% specificity in 20 strains which showed resistance to 500 μg of gentamicin per ml. We recommend this procedure be adopted as a routine screening test to detect high-level aminoglycoside resistance in enterococci.

Original languageEnglish (US)
Pages (from-to)2443-2444
Number of pages2
JournalJournal of clinical microbiology
Volume25
Issue number12
DOIs
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)

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