Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci

M. J. Zervos; J. E. Patterson; S. Edberg; C. Pierson; C. A. Kauffman; T. S. Mikesell; D. R. Schaberg

doi:10.1128/jcm.25.12.2443-2444.1987

Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci

M. J. Zervos, J. E. Patterson, S. Edberg, C. Pierson, C. A. Kauffman, T. S. Mikesell, D. R. Schaberg

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Growth in a single broth microdilution well containing gentamicin at a concentration of 500 μg/ml predicted high-level resistance to gentamicin (MIC, ≥ 2,000 μg/ml) in 505 of 508 clinical isolates of enterococci. Failure to achieve synergistic killing with the combination of penicillin and an aminoglycoside was demonstrated with 100% specificity in 20 strains which showed resistance to 500 μg of gentamicin per ml. We recommend this procedure be adopted as a routine screening test to detect high-level aminoglycoside resistance in enterococci.

Original language	English (US)
Pages (from-to)	2443-2444
Number of pages	2
Journal	Journal of clinical microbiology
Volume	25
Issue number	12
DOIs	https://doi.org/10.1128/jcm.25.12.2443-2444.1987
State	Published - 1987
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

Access to Document

10.1128/jcm.25.12.2443-2444.1987

Cite this

@article{9fa18bd33e90474f97a9e6b7e022f9bc,

title = "Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci",

abstract = "Growth in a single broth microdilution well containing gentamicin at a concentration of 500 μg/ml predicted high-level resistance to gentamicin (MIC, ≥ 2,000 μg/ml) in 505 of 508 clinical isolates of enterococci. Failure to achieve synergistic killing with the combination of penicillin and an aminoglycoside was demonstrated with 100% specificity in 20 strains which showed resistance to 500 μg of gentamicin per ml. We recommend this procedure be adopted as a routine screening test to detect high-level aminoglycoside resistance in enterococci.",

author = "Zervos, {M. J.} and Patterson, {J. E.} and S. Edberg and C. Pierson and Kauffman, {C. A.} and Mikesell, {T. S.} and Schaberg, {D. R.}",

year = "1987",

doi = "10.1128/jcm.25.12.2443-2444.1987",

language = "English (US)",

volume = "25",

pages = "2443--2444",

journal = "Journal of clinical microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "12",

}

TY - JOUR

T1 - Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci

AU - Zervos, M. J.

AU - Patterson, J. E.

AU - Edberg, S.

AU - Pierson, C.

AU - Kauffman, C. A.

AU - Mikesell, T. S.

AU - Schaberg, D. R.

PY - 1987

Y1 - 1987

N2 - Growth in a single broth microdilution well containing gentamicin at a concentration of 500 μg/ml predicted high-level resistance to gentamicin (MIC, ≥ 2,000 μg/ml) in 505 of 508 clinical isolates of enterococci. Failure to achieve synergistic killing with the combination of penicillin and an aminoglycoside was demonstrated with 100% specificity in 20 strains which showed resistance to 500 μg of gentamicin per ml. We recommend this procedure be adopted as a routine screening test to detect high-level aminoglycoside resistance in enterococci.

AB - Growth in a single broth microdilution well containing gentamicin at a concentration of 500 μg/ml predicted high-level resistance to gentamicin (MIC, ≥ 2,000 μg/ml) in 505 of 508 clinical isolates of enterococci. Failure to achieve synergistic killing with the combination of penicillin and an aminoglycoside was demonstrated with 100% specificity in 20 strains which showed resistance to 500 μg of gentamicin per ml. We recommend this procedure be adopted as a routine screening test to detect high-level aminoglycoside resistance in enterococci.

UR - http://www.scopus.com/inward/record.url?scp=0023502433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023502433&partnerID=8YFLogxK

U2 - 10.1128/jcm.25.12.2443-2444.1987

DO - 10.1128/jcm.25.12.2443-2444.1987

M3 - Article

C2 - 3123515

AN - SCOPUS:0023502433

SN - 0095-1137

VL - 25

SP - 2443

EP - 2444

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

IS - 12

ER -

Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this