Single-cell omics: Circulating tumor cells

Paul Winograd; Benjamin Di Pardo; Colin M. Court; Shonan Sho; James S. Tomlinson

doi:10.1016/B978-0-12-817532-3.00003-7

Single-cell omics: Circulating tumor cells

Paul Winograd
, Benjamin Di Pardo
, Colin M. Court
, Shonan Sho
, James S. Tomlinson

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Scopus citations

Abstract

This chapter presents circulating tumor cells (CTCs) as a real-time liquid biopsy, with an emphasis on single-cell sequencing. Following an abbreviated introduction of CTC biology, a variety of the most frequently utilized CTC enrichment and detection strategies is summarized. The focus of the chapter then shifts toward single-cell CTC analysis. Methods for single CTC isolation and molecular profiling are reviewed, followed by a summary of several single CTC genomic and transcriptomic studies performed in various malignancies. The chapter closes with a discussion on the importance of single CTC molecular analysis and the unique challenges in the field.

Original language	English (US)
Title of host publication	Single-Cell Omics
Subtitle of host publication	Volume 2: Application in Biomedicine and Agriculture
Publisher	Elsevier
Pages	37-54
Number of pages	18
ISBN (Electronic)	9780128175323
DOIs	https://doi.org/10.1016/B978-0-12-817532-3.00003-7
State	Published - Jan 1 2019
Externally published	Yes

Keywords

Biomarkers
CTC enrichment
CTC isolation
Circulating tumor cells (CTCs)
Epithelial-mesenchymal transition
Liquid biopsy
Single-Cell genomic sequencing
Single-Cell transcriptome analysis

ASJC Scopus subject areas

General Economics, Econometrics and Finance
General Business, Management and Accounting

Access to Document

10.1016/B978-0-12-817532-3.00003-7

Cite this

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title = "Single-cell omics: Circulating tumor cells",

abstract = "This chapter presents circulating tumor cells (CTCs) as a real-time liquid biopsy, with an emphasis on single-cell sequencing. Following an abbreviated introduction of CTC biology, a variety of the most frequently utilized CTC enrichment and detection strategies is summarized. The focus of the chapter then shifts toward single-cell CTC analysis. Methods for single CTC isolation and molecular profiling are reviewed, followed by a summary of several single CTC genomic and transcriptomic studies performed in various malignancies. The chapter closes with a discussion on the importance of single CTC molecular analysis and the unique challenges in the field.",

keywords = "Biomarkers, CTC enrichment, CTC isolation, Circulating tumor cells (CTCs), Epithelial-mesenchymal transition, Liquid biopsy, Single-Cell genomic sequencing, Single-Cell transcriptome analysis",

author = "Paul Winograd and \{Di Pardo\}, Benjamin and Court, \{Colin M.\} and Shonan Sho and Tomlinson, \{James S.\}",

year = "2019",

month = jan,

day = "1",

doi = "10.1016/B978-0-12-817532-3.00003-7",

language = "English (US)",

pages = "37--54",

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TY - CHAP

T1 - Single-cell omics

T2 - Circulating tumor cells

AU - Winograd, Paul

AU - Di Pardo, Benjamin

AU - Court, Colin M.

AU - Sho, Shonan

AU - Tomlinson, James S.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - This chapter presents circulating tumor cells (CTCs) as a real-time liquid biopsy, with an emphasis on single-cell sequencing. Following an abbreviated introduction of CTC biology, a variety of the most frequently utilized CTC enrichment and detection strategies is summarized. The focus of the chapter then shifts toward single-cell CTC analysis. Methods for single CTC isolation and molecular profiling are reviewed, followed by a summary of several single CTC genomic and transcriptomic studies performed in various malignancies. The chapter closes with a discussion on the importance of single CTC molecular analysis and the unique challenges in the field.

AB - This chapter presents circulating tumor cells (CTCs) as a real-time liquid biopsy, with an emphasis on single-cell sequencing. Following an abbreviated introduction of CTC biology, a variety of the most frequently utilized CTC enrichment and detection strategies is summarized. The focus of the chapter then shifts toward single-cell CTC analysis. Methods for single CTC isolation and molecular profiling are reviewed, followed by a summary of several single CTC genomic and transcriptomic studies performed in various malignancies. The chapter closes with a discussion on the importance of single CTC molecular analysis and the unique challenges in the field.

KW - Biomarkers

KW - CTC enrichment

KW - CTC isolation

KW - Circulating tumor cells (CTCs)

KW - Epithelial-mesenchymal transition

KW - Liquid biopsy

KW - Single-Cell genomic sequencing

KW - Single-Cell transcriptome analysis

UR - https://www.scopus.com/pages/publications/85082088834

UR - https://www.scopus.com/pages/publications/85082088834#tab=citedBy

U2 - 10.1016/B978-0-12-817532-3.00003-7

DO - 10.1016/B978-0-12-817532-3.00003-7

M3 - Chapter

AN - SCOPUS:85082088834

SP - 37

EP - 54

BT - Single-Cell Omics

PB - Elsevier

ER -

Single-cell omics: Circulating tumor cells

Abstract

Keywords

ASJC Scopus subject areas

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