Abstract
This chapter presents circulating tumor cells (CTCs) as a real-time liquid biopsy, with an emphasis on single-cell sequencing. Following an abbreviated introduction of CTC biology, a variety of the most frequently utilized CTC enrichment and detection strategies is summarized. The focus of the chapter then shifts toward single-cell CTC analysis. Methods for single CTC isolation and molecular profiling are reviewed, followed by a summary of several single CTC genomic and transcriptomic studies performed in various malignancies. The chapter closes with a discussion on the importance of single CTC molecular analysis and the unique challenges in the field.
Original language | English (US) |
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Title of host publication | Single-Cell Omics |
Subtitle of host publication | Volume 2: Application in Biomedicine and Agriculture |
Publisher | Elsevier |
Pages | 37-54 |
Number of pages | 18 |
ISBN (Electronic) | 9780128175323 |
DOIs | |
State | Published - Jan 1 2019 |
Externally published | Yes |
Keywords
- Biomarkers
- CTC enrichment
- CTC isolation
- Circulating tumor cells (CTCs)
- Epithelial-mesenchymal transition
- Liquid biopsy
- Single-Cell genomic sequencing
- Single-Cell transcriptome analysis
ASJC Scopus subject areas
- General Economics, Econometrics and Finance
- General Business, Management and Accounting