Single-cell library preparation of iPSC-derived neural stem cells

Jeffrey Kim, Marcel M. Daadi

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Single-cell RNA-seq technology allows for the identification of heterogeneous cell populations, measures stochastic gene expressions, and identifies highly variable genes. Thus, with this technology it is possible to identify relevant pathways involved in development or in disease progression. Herein, we describe a protocol to capture and process single-cell transcriptomes that will be used for RNA sequencing. This chapter discusses the use of the Fluidigm C1 System and Integrated Fluidic Circuit microfluidics system, TapeStation 4200, SMART-Seq v4, Nextera XT Library Preparation Kit, and AMPure XP beads.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages129-143
Number of pages15
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1919
ISSN (Print)1064-3745

Keywords

  • C1
  • Fluidigm
  • Library preparation
  • Neural stem cells
  • Next-generation sequencing
  • Nextera XT
  • RNA-seq
  • Single cell
  • Single-cell RNA-seq
  • SMART-Seq

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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