TY - JOUR
T1 - Simultaneous Determination of N-Acetyltransferase Activity, Hydroxyindole-O-methyl-transferase Activity, and Melatonin Content in the Pineal Gland of the Syrian Hamster
AU - Steinlechner, Stephan
AU - Champney, Thomas H.
AU - Houston, Marshall L.
AU - Reiter, Russel J.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1984/1
Y1 - 1984/1
N2 - The activities of serotonin N-acetyltransferase (NAT) and hydroxyindole-O-methyltransferase (HIOMT) and the melatonin content were measured in Syrian hamster pineal glands at 2-hr intervals over a period of 24 hr. NAT and HIOMT are the two enzymes which catalyze the formation of melatonin from serotonin. The use of micromethods for determination of the enzyme activities allowed concurrent measurement of NAT and melatonin or HIOMT and melatonin in the same gland. HIOMT activity showed no significant diurnal rhythm whereas NAT activity and melatonin content exhibited distinct peak values late in the dark phase as described previously. Despite an apparent parallelism between the NAT activity rhythm and melatonin content, no correlation exists between these parameters in single pineal glands.
AB - The activities of serotonin N-acetyltransferase (NAT) and hydroxyindole-O-methyltransferase (HIOMT) and the melatonin content were measured in Syrian hamster pineal glands at 2-hr intervals over a period of 24 hr. NAT and HIOMT are the two enzymes which catalyze the formation of melatonin from serotonin. The use of micromethods for determination of the enzyme activities allowed concurrent measurement of NAT and melatonin or HIOMT and melatonin in the same gland. HIOMT activity showed no significant diurnal rhythm whereas NAT activity and melatonin content exhibited distinct peak values late in the dark phase as described previously. Despite an apparent parallelism between the NAT activity rhythm and melatonin content, no correlation exists between these parameters in single pineal glands.
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U2 - 10.3181/00379727-175-41773
DO - 10.3181/00379727-175-41773
M3 - Article
C2 - 6538043
AN - SCOPUS:0021348793
SN - 0037-9727
VL - 175
SP - 93
EP - 97
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 1
ER -