Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils

Michio Nakamura; Charles R. Baxter; Bettie Sue S. Masters

doi:10.1016/0006-291X(81)91175-X

Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils

Michio Nakamura, Charles R. Baxter, Bettie Sue S. Masters

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Phagocytosis-connected oxygen consumption by human neutrophils and corresponding NAD(P)H oxidase were measured by an oxygen electrode with sequential additions of opsonized zymosan, Renex 30 (0.067%), and NAD(P)H. At a concentration of 0.15 mM substrate, NADPH oxidase activity of stimulated neutrophils was twice that required to account for accompanying oxygen consumption, and was about 20 times higher than that activity obtained from resting cells. NADH oxidase activity of phagocytizing cells, however, was negligible at the same concentration of substrate. With high recovery of oxidase activity, these results strongly suggest that NADPH is the dominant electron donor to oxygen in phagocytizing human neutrophils.

Original language	English (US)
Pages (from-to)	743-751
Number of pages	9
Journal	Biochemical and Biophysical Research Communications
Volume	98
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(81)91175-X
State	Published - Feb 12 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Nakamura, M., Baxter, C. R., & Masters, B. S. S. (1981). Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils. Biochemical and Biophysical Research Communications, 98(3), 743-751. https://doi.org/10.1016/0006-291X(81)91175-X

Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity : Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils. / Nakamura, Michio; Baxter, Charles R.; Masters, Bettie Sue S.

In: Biochemical and Biophysical Research Communications, Vol. 98, No. 3, 12.02.1981, p. 743-751.

Research output: Contribution to journal › Article › peer-review

Nakamura, M, Baxter, CR & Masters, BSS 1981, 'Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils', Biochemical and Biophysical Research Communications, vol. 98, no. 3, pp. 743-751. https://doi.org/10.1016/0006-291X(81)91175-X

Nakamura M, Baxter CR, Masters BSS. Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils. Biochemical and Biophysical Research Communications. 1981 Feb 12;98(3):743-751. https://doi.org/10.1016/0006-291X(81)91175-X

Nakamura, Michio ; Baxter, Charles R. ; Masters, Bettie Sue S. / Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity : Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils. In: Biochemical and Biophysical Research Communications. 1981 ; Vol. 98, No. 3. pp. 743-751.

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title = "Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils",

abstract = "Phagocytosis-connected oxygen consumption by human neutrophils and corresponding NAD(P)H oxidase were measured by an oxygen electrode with sequential additions of opsonized zymosan, Renex 30 (0.067%), and NAD(P)H. At a concentration of 0.15 mM substrate, NADPH oxidase activity of stimulated neutrophils was twice that required to account for accompanying oxygen consumption, and was about 20 times higher than that activity obtained from resting cells. NADH oxidase activity of phagocytizing cells, however, was negligible at the same concentration of substrate. With high recovery of oxidase activity, these results strongly suggest that NADPH is the dominant electron donor to oxygen in phagocytizing human neutrophils.",

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Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: Direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils

Abstract

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