TY - JOUR
T1 - Simple method for detecting fluconazole-resistant yeasts with chromogenic agar
AU - Patterson, Thomas F.
AU - Revankar, Sanjay G.
AU - Kirkpatrick, William R.
AU - Dib, Olga
AU - Fothergill, Annette W.
AU - Redding, Spencer W.
AU - Sutton, Deanna A.
AU - Rinaldi, Michael G.
PY - 1996/7
Y1 - 1996/7
N2 - A method for detecting fluconazole-resistant yeasts was developed that uses chromogenic agar containing fluconazole. Yeasts were plated on media with fluconazole at 0, 8, and 16 μg/ml. On media without fluconazole, normal growth of susceptible yeasts (defined as those having a fluconazole MIC of <8 μg/ml) was detected, while fluconazole-containing media suppressed susceptible strains and normal colonies of resistant yeasts (fluconazole MICs of ≥8 μg/ml) were detected. This method was used to screen for resistance in oropharyngeal candidiasis. Isolates having fluconazole MICs of ≥8 μg/ml and <8 μg/ml were correctly predicted in 43 of 45 cultures and 115 of 116 cultures, respectively. This screening method appears to be rapid and sensitive for detection of fluconazole-resistant yeasts.
AB - A method for detecting fluconazole-resistant yeasts was developed that uses chromogenic agar containing fluconazole. Yeasts were plated on media with fluconazole at 0, 8, and 16 μg/ml. On media without fluconazole, normal growth of susceptible yeasts (defined as those having a fluconazole MIC of <8 μg/ml) was detected, while fluconazole-containing media suppressed susceptible strains and normal colonies of resistant yeasts (fluconazole MICs of ≥8 μg/ml) were detected. This method was used to screen for resistance in oropharyngeal candidiasis. Isolates having fluconazole MICs of ≥8 μg/ml and <8 μg/ml were correctly predicted in 43 of 45 cultures and 115 of 116 cultures, respectively. This screening method appears to be rapid and sensitive for detection of fluconazole-resistant yeasts.
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U2 - 10.1128/jcm.34.7.1794-1797.1996
DO - 10.1128/jcm.34.7.1794-1797.1996
M3 - Article
C2 - 8784592
AN - SCOPUS:0030014760
SN - 0095-1137
VL - 34
SP - 1794
EP - 1797
JO - Journal of clinical microbiology
JF - Journal of clinical microbiology
IS - 7
ER -